Basophils of Pars Distalis Adenohypophysis in Madras Red Sheep (Ovis aries) – Histology with Reference to Various Age Groups
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Date
2017-02
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Abstract
Histophysiological study of pituitary gland was conducted on Madras red sheep to
record the age related changes. The pars distalis adenohypophysis comprised of cells
arranged in irregular cords, grouped as acidophils, basophils and chromophobes. The
basophils were round, oval and irregularly polygonal cells with distinct cell boundaries
observed as 4 variants. The type I basophils were angular or polygonal cells stained intensely
with aniline blue both in Mallory’s triple stain and Mallory’s Azan technique. Type II
basophils were oval, polygonal in shape packed with course granules and showed strong PAS
reaction. Type III basophils were oval or round cells, purple to violet in acid fuchsin and
aniline blue preparations. These were aldehyde fuchsin negative and showed a mild PAS
reactivity as compared to type II basophils. Type IV basophils were irregularly oval to
elongate in shape with their processes extended through the neighbouring cells, moderately
PAS positive but strongly positive for lead Haematoxylin. The number of basophils ranged
from 862 ± 52.98 cells/mm2 in lactating animals upto 1428 ± 83.14 cells/mm2 in pubertal
animals. The increased basophils during pubertal age groups of sheep indicated that the
activity of FSH and LH cell that contributed the hormones required for the estrous cycles.
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Veterinary Science, Veterinary Anatomy