Effect of rhizobium and rhizosphere extract on in-vitro regenerated plantlets of chickpea (Cicer arietinum L.)
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Date
2007
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Publisher
CCSHAU
Abstract
Experiments were conducted to improve transplantation
efficiency of in-vitro regenerated plantlets of chickpea. Three chickpea cultivars
C 235, HC 5 and HC 1 were cultured for shoot regeneration and subsequently
for root regeneration.
A total of five Rhizobium strains were screened for IAA production out of
which Strain M 113 was found to secrete maximum IAA (2.4 μg/ml) whereas
strain M 25 showed a maximum IAA level of 2.2 (μg/ml) in culture. Rhizobium
supplemented culture medium helped in better survival of transplanted
explants in comparison to regenerated plantlets on normal control medium.
Rhizobium consortium treatment and adding Rhizobium culture over roots did
not help in rooting and survival of the regenerants. Instead of it adding culture
extract in the rooting medium with MS salts helped a lot in improving survival.
Among MRC 113, MRC 25 and MRC 104 varietal response shown was
maximum in cultivar C235 where it was 90% and it was minimum in cultivar
HC 1 where it was 66%. In MRC 113, MRC 25 and MRC 104 no growth hormones were added but only Rhizobium culture was added showing that
culture is having plant growth promotion abilities, which may be due to
phytohormone IAA production in culture. Cultivar C 235 gave better response
in comparison to other cultivars on MRC 113 proving that Rhizobium strain
113 is best plant growth promoting among these strains. In medium MSE no
rooting hormones were added but still it showed good rooting quality showing
that rhizosphere extract may also promote root development and hence rooting
quality.
Rhizobia proved promising in improving survival of regenerated
chickpea. This line of treatment needs to be further refined to enable seed set
in the regenerated plants. Other plant growth promoting microbes in
combination with Rhizobium inoculation are further expected to improve the
response.
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Keywords
Chickpea, Regeneration, Rhizobium, Transplantation, Plant growth promotion