Evaluation of EPN formulations, their shelf life and efficacy of heterorhabditis indica (RCR) (Heterorhabditidae: Nematoda) against economically important
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Date
2010
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UAS Dharwad
Abstract
Investigations were carried out on shelf life of different nematode formulations of
locally isolated EPN Heterorhabditis indica (RCR), to standardize the density and
temperature for long storage period and bio efficacy of different nematode formulations
against economically important insect pests was undertaken under laboratory and field
conditions during 2005-08 at the Department of Entomology, College of Agriculture,
Raichur.
The studies revealed that in aqueous formulation the best combination of
temperature, population density and duration of storage was 10°C, 10000 IJs/ml and 120 days
of storage period, respectively. The corresponding values for talc formulation found to be
20ºC, 10000 IJs/ml and 120 days of storage period. Similar studies in sponge and alginate
formulations also showed encouraging results.
The production and duration of emergence studies revealed that one to four week old
IJs stored at 10 and 15 ºC was found to be most appropriate for maximum production in
aqueous and alginate formulations and time taken for emergence was shorter.
Among the different formulations aqueous formulation revealed maximum larval
mortality of H.armigera when exposed to 90 days old IJs stored at lower temperatures.
Maximum larval mortality of S. litura was recorded from aqueous (spray) and alginate (bait)
formulations when exposed to 30, 60 and 90 days old IJs stored at lower temperatures.
Efficacy of different nematode formulations evaluated against H. armigera in
chickpea ecosystem revealed that the plots treated with talc formulation @ 8000 and 10,000
IJs/g recorded maximum yield with minimum pod damage.
Among the various formulations evaluated against C. partellus infesting sorghum, the
aqueous form of whorl application was found to be superior followed by talc and spray form
of application.