Studies on influence of provenances, seed priming and seed storage in hybrid maize (Zea mays L.)

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Date
2013-10-23
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University of Agricultural Sciences, Bengaluru
Abstract
The field experiment was conducted at three different provenances viz., Mandya, Hassan and Chickballapur with two Maize hybrids Hema and Nithyashree with three fertilizer levels during kharif 2011 and 2012 and the laboratory study on seed priming and seed storage was conducted at GKVK, Bangalore during the same period. Among provenances, Mandya (southern dry zone) is more suitable provenance for seed production of Hema with the application of more than 50% RDF (225:112:60kg NPK ha-1) to obtain higher growth and seed yield parameters like plant height (193.10cm), time to 50% flowering (47.74days), cob length (20.33cm), seeds per cob (593) and seed yield (77.08qha-1); seed quality parameters like germination percentage (99%), SVI-I (3767) and low electrical conductivity (0.106dSm-1) compared to Nithyashree hybrid with lower seed yield (34.75qha-1), SVI-I (3136) and high electrical conductivity (0.154dSm-1). Seeds subjected for natural ageing for 12 months and accelerated ageing at different temperature and relative humidity levels for 12 days with an interval of three days at Bangalore conditions. At the end of three day accelerated ageing, reduced germination percentage (100 to 93%), total soluble proteins (93 to 80μg g-1), amylase activity (0.343 to 0.300mg maltose released min-1mg-1), DNA content (60 to 46ngg-1 of seed) and increased electrical conductivity (0.106 to 0.230dSm-1) observed. The reduction in quality parameters of three day of accelerated aged seeds are equivalent to twelve months naturally aged seeds. Therefore accelerated ageing test can be used to predict the storability of maize by observing the molecular and biochemical changes. Seed lots having low and high vigour (<70 and >90% germination respectively) primed with different priming chemicals and kept in different temperatures to improve the seed quality in low vigour seeds and observed that the seed quality parameters were improved by priming with KH2PO4 (1%) for fourteen hours at ambient temperature (28ºC).
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Keywords
sowing, germinability, maize, fertilizers, enzymes, hybrids, proteins, relative humidity, planting, drying
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