Studies on Yellow Mosaic Disease on Mungbean [Vigna radiata (L.) Wilczek]
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Date
2017-07
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University of Agricultural Science, Dharwad
Abstract
A study on Mungbean yellow mosaic virus (MYMV) disease involving survey of four districts (Belagavi, Dharwad, Haveri, Gadag) in kharif 2016 and summer 2017, molecular characterization, host range, seed transmission, screening of advanced breeding lines and disease management was carried out during 2016-2017. During kharif 2016, the highest incidence (16.12 %) was in Haveri and the least (11.7 %) was in Gadag. During summer 2017, highest incidence (22.54 %) was in Dharwad and the least (20.73 %) was in Belagavi.
The coat protein gene of MYMV (642 bp) was amplified using specific primers. PCR amplification, sequencing and construction of phylogenetic tree showed that MYMV Dharwad isolate was 99 per cent homology with different isolates of Horsegram yellow mosaic virus (HYMV). Therefore, MYMV of Dharwad isolate is the close relative of HYMV.
MYMV was transmitted to seven crop plants except bhendi namely soybean, pigeonpea, blackgram, horsegram, frenchbean, cowpea, fieldbean and three weed hosts viz., Ageratum conyzoides, Corchorus trilocularis and Sida acuta and these hosts acts as collateral hosts. Studies revealed that MYMV was present in MYMV infected seeds and detected seed borne nature.
Twenty-one of 24 advanced genotypes screened against MYMV during summer 2016 showed resistant reaction.
Among different modules tested for the management of Mungbean yellow mosaic disease, adoptive module (M3) including seed treatment with Rhizobium (5 g/kg), border crop of maize (3 lines), yellow sticky trap (20/ha), Verticillium lecani (5 g/l), Triazophos (1.5 ml/l) at 30 DAS and spray of neem oil (5 ml/l) at 45 DAS recorded least disease incidence (8.94 %) and lowest whitefly population (2.20 whiteflies) with highest yield of 11.61 q/ha with B: C ratio of 3.00. Hence, adoptive module is best for management of yellow mosaic disease.