Identification of pearl millet [Pennisetum glaucum (L.) R. Br.] hybrids and their parents using SSR markers
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Date
2017
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CCSHAU
Abstract
Pearl millet [Pennisetum glaucum (L.) R. Br.] (2n=14) is a short life span, highly crosspollinated,
diploid, photo-synthetically efficient C4 monocot belonging to family Poaceae. It is
primarily grown as a rain fed crop in the low rainfall zones of Sub-Saharan Africa and Indian
subcontinent. Since 1986, 67 cultivars based on ICRISAT-bred germplasm (14 OPVs and 53 hybrids)
have been released and notified in India. The improved cultivars are grown on more than 4.5 million ha
(of about 9 million ha), of which the OPVs cover only 0.6 to 0.8 million ha, with the remaining area
cultivated with hybrids. Determining the genetic purity of hybrid seed is an essential requirement for its
commercial use. The SSR markers are preferred molecular markers for genetic purity identification due
to their high efficiency, co-dominance, reproducibility, simplicity and accessibility to laboratories.
Total six pearl millet hybrids along with their parents were used in this study. Genomic DNA of pearl
millet hybrids and their parents (HHB67-Improved ,ICMA 843-22, ICMB 843-22, H 77/833-2-
202,HHB146,ICMA 95222, ICMB 95222, HTP 94/54, HHB94,ICMA 89111, ICMB 89111, G 73-107,
HB256,ICMA 94222, ICMB 94222, H 1305, HHB216,HMS 37A, HMS 37B, HTP 03/13, HHB117,
HMS 7A HMS 7B 77/29-2) was isolated using CTAB method. Out of 84 SSR primer pairs used, 7 SSR
primers could distinguish the different hybrids and their parents. The SSR data was also used to
determine genetic relationship among these genotypes. This study would a great help for testing purity
of these hybrids.
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