Histological and histochemical studies on the small intestine of domestic pig (Sus scrofa domesticus)
Loading...
![Thumbnail Image](assets/images/Item.jpg)
Date
2023-06-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
LUVAS Hisar
Abstract
Ten pigs of 8 to 10 months of age were used to study the histology and histochemistry of the
small intestine. The villi were of different shapes and sizes were present throughout the length of small
intestine. They were short and leaf shaped in initial portion of the intestine but gradually increased in
height and was finger shaped towards the jejunum and again gradually decreased in height towards the
ileo-caecal junction. The epithelium was simple columnar with goblet cells. The number of goblet cells
increased towards ileum. Histochemical studies of the goblet cells in the epithelium showed presence
of glycogen and both acidic and neutral mucopolysaccharides but there was predominance of acidic
mucopolysaccharides. Hyaluronic acid and sialmucins were also demonstrated in goblet cells by Alcian
blue stain (pH 2.5). The enterochromaffin cells were present in all segments of intestine and there
number was more in cranial duodenum.. The intestinal glands were simple tubular gland lined with
columnar epithelium and were consisted of columnar, goblet, enterochromaffin cells. The intestinal
glands showed presence of glycogen and both acidic and neutral mucopolysaccharides but there was
predominance of acidic one especially in goblet cells. The activity towards the Alcian Blue was also
strong in intestinal glands of the small intestine. The Brunner’s glands were tubulo-alveolar and mucus
in nature. They were present in submucosa of duodenum and there number was decreased from cranial
to caudal portion of duodenum. Histochemical studies of Brunner’s glands showed moderate to strong
reaction with the PAS but the reaction was strong towards the luminal surface for neutral
mucosubstance. Brunner’s glands also showed weak activity towards alcian blue stain. The lymphoid
nodules were present on the anti-mesenteric part of the intestine. These lymphatic nodules were locate
from caudal jejunum to all segments of ileum. The lymphatic nodules or Peyer’s patches were of
various shapes and sizes. Their shape varied from round to oval but their size mostly small to medium.
The Peyer’s patches along with follicle associated epithelium (FAE) were observed in caudal jejunum
and entire ileum. The lymphatic nodules were having lightly germial center packed with lymphocytes,
lymphoblasts, macrophages and plasma cells. The darkly stained peripheral zone called corona was
having densely packed lymphocytes.