Evaluation of Post Harvest Quality of Pointed Gourd (Trichosanthes dioica Roxb.) Genotypes during Storage

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Date
2022
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DRPCAU, PUSA
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The present study entitled “Evaluation of Post Harvest Quality of Pointed Gourd (Trichosanthes dioica Roxb.) Genotypes during Storage” was carried using 12-pointed gourd crop of the genotypes/varieties namely „PGS-1‟, „PGS-2‟, „PGS-3‟, „PGS-5‟, „PGS-11‟, „PGS-12‟, „PGS-13‟, „Swarna Alaukik‟, „Swarna Rekha‟, „Swarna Suruchi‟, „Kashi Alankar‟, „Kashi Suphal‟ cultivated during the Zaid season of 2021–2022 at research laboratory of TCA, Dholi, Botany Plant Physiology and Biochemistry and National Research Centre on Litchi, Bihar. Pointed gourd considered as the „King of gourd‟ in India. There are 42 species found in Indo-malayan region and 22 are found in India. Among the several species of Trichosanthes but the Trichosanthes dioica bears commercial value. Several cultivars (genotypes) have been developed by the horticulturists and few varieties (genotypes) are added every year. However, there is lack of postharvest research on biochemical/nutritional profiling and phytochemical changes during storage which is yet to be attempted in the genotypes. Hence, present study was conducted with two objectives, these were in objective I divided into two experiments, in experiment No - I, 12 different genotypes were assessed for physical, physiological, quality, functional Name of Student : SHUBHAM MAURYA Registration Number : M/HORT. (VS)/489/2020-21 Major Advisor : Dr. K. Prasad Degree to be Awarded : Master of Science in Agriculture (Horticulture) Vegetable Science Department : Horticulture Major Subject : Vegetable Science Minor Subject : Plant Breeding and Genetics Year : 2021-22 Title of Thesis : Evaluation of Post Harvest Quality of Pointed gourd (Trichosanthes dioica Roxb.) Genotypes during Storage University : Dr. Rajendra Prasad Central Agricultural University, Pusa (Samastipur), Bihar 848125 Total Pages of Thesis : 86 + Bibliography (i-vii) and enzymatic activities, and in the experiment II, 12 genotypes were assessed for iochemical and enzymatic activities changes during storage at am ient storage condition 25 4 C and 65 5% RH). Under the objective II, the suitability of genotypes for sweet and dehydrated product making from the selected genotypes was explored. In context of first objective for the nutritional characterisation of selected pointed gourd genotypes, fruits of 12 genotypes were manually harvested, washed and stored, recordings were taken on various postharvest attributes. Our results revealed that the selected genotypes varied significantly for the studied attributes such as physical, physiological, quality, functional, enzymatic and minerals content. In context to physical parameters. Among the selected 12 genotypes fruit length (10.17 cm), width (3.53 cm) and weight (32.78 g) was the maximum in „PGS-3‟. Fruit firmness was the highest in „PGS-2‟ 13.20 N). The physiological activity i.e., respiration rate was the highest in „Swarna Alaukik‟ 54 ml CO2 kg-1 h-1). Among the biochemical attributes, the highest total soluble solids were the maximum in „Swarna Alaukik‟ 3.50 ºB). The titrata le acidity 0.60 %), ascorbic acid (29.50 mg 100g-1), dietary fibre (5.20 mg 100 g-1), was the maximum in „PGS-3‟. The total sugars content was the maximum in „Swarna Alaukik‟ 1.70 %). The total phenolic content (117.40 μg GAE g-1), total flavonoids content (1.08 mg 100 g-1), chlorophyll „a‟ 0.299 mg 100 g-1) and chlorophyll „ ‟ 0.144 mg 100 g-1) was the maximum in „PGS-3‟. Furthermore, the total carotenoids content was the maximum in „Swarna Alauik‟ 0.016 mg 100 g-1). The maximum values of DPPH free radical scavenging activity and were the observed in „PGS-11‟ 58.23 μmol TE g-1). The genotype „PGS-2‟ exhi ited highest polygalactouronsae 40.33 μg galactouronic acid g-1 h-1 FW) and pectin methylesterase activity (0.07 μmol min-1 g-1 FW). However, the LOX activity was the maximum in „PGS-12‟ 0.003 μmol min-1 ml-1). Among the studied mineral content of genotypes, phosphorus content was the maximum in „Kashi Suphal‟ 42 mg 100 g-1) while the potassium content was the maximum in „Swarna Suruchi‟ 86 mg 100 g-1). Under experiment II of the first objective, where the quality changes during storage of selected genotypes, in context to physical attribute, physiological attribute, irrespective of storage days, the fruit firmness was the highest in „PGS-2‟ 10.84 N). Irrespective of storage day, the PLW was the maximum in „Swarna Alaukik‟ 16.27 %). The highest respiration rate was exhi ited y „Swarna Alaukik‟ 71.00 ml CO2 kg-1 h-1). The total solu le solids content was the highest in „Swarna Alaukik‟ (4.09 B). The total phenolic content was the maximum in „Swarna Suruchi‟ 97.85 μg GAE g-1), the highest titratable acidity (0.56 %), the total flavonoid content (0.92 mg 100 g-1), the chlorophyll „a‟ content 0.275 mg 100 g-1), the chlorophyll „ ‟ content (0.122 mg 100 g-1), was the maximum in „PGS-3‟. The highest ascor ic acidity was recorded in „PGS-11‟ 28.07 mg 100 g-1). The total sugar (1.89 %), the carotenoid content (0.036 mg 100 g-1) was the maximum in „Swarna Alauik‟ and the DPPH free radical scavenging activity content was highest in „PGS-11‟ 33.53 μmol trolox g-1). In context of enzymatic activity, the highest polygalactouronsae activity was observed in „Swarna Alaukik‟ 45.66 μg galactouronicacid g-1 h-1 FW). The highest pectin methylestearase activity was observed in „PGS-2‟ 0.16 μmol min-1 g-1 FW). The highest LOX activity „Kashi Suphal‟ 0.003256 μmol min-1 ml-1). In context of second objective product making, the genotypes, „Swarna Alaukik‟, „PGS-1‟, „PGS- 13‟ and „Kashi Suphal‟ displayed greatest suitability for pointed gourd sweet and „PGS-3‟, „Swarna Rekha‟, „Swarna Suruchi‟ and „PGS-11‟ were highly suitable for dehydrated pointed gourd.
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