Studies on Anestrus in Murrah Buffaloes During Summer and Response to Insulin and Dextrose Pretreatment in Ovsynch Protocol
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Date
2009
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Chhattisgarh Kamdhenu Vishwavidyalaya, Durg (C.G.)
Abstract
The experimental study was conducted on one hundred and eight Murrah buffaloes. The objective was to study the incidence of true anestrus during summer, hormonal and mineral profile in cyclic and acyclic Murrah buffaloes during summer, efficacy of insulin and dextrose pretreatment to GnRH on induction of cyclicity, and conception in Murrah buffaloes during summer. For study of incidence of anestrus 108 Murrah buffaloes were per rectally examined among which six cyclic and twenty four acyclic buffaloes were selected for the estimation of blood glucose, NEFA, mineral (Ca, Pi, Mg Ca: Pi, Zn, Cu, Fe, Co, Mn and I2) and hormonal (progesterone, estrogen and insulin) profile in blood sample collected on day 0 (i.e. prior to any treatment) during summer. The twenty four acyclic true anestrous buffaloes were subsequently divided into four treatment groups. The treatment instituted was: Ovsynch protocol (intramuscular inj. of GnRH, PGF2α and GnRH on day 0, 7 and 9 respectively; Group-I), subcutaneous inj. of insulin (Human- Mixtard) on day 6, 7 and 8 in addition to treatment given in Group-I (Group-II), intravenous inj. of dextrose (Woktose-25) on day 6, 7 and 8 in addition to treatment given in Group-I (Group-III) and Group-IV as a control group without any treatment. The estimation of hormones (progesterone, estrogen and insulin) and glucose was carried out in the blood sample collected on day 0 (i.e. prior to GnRH), day 7( i.e. prior to PGF2α) and day 9 (i.e. prior to second GnRH). The incidence of true anestrus, silent estrus, overt estrus and buffaloes found to be in estrus while per rectal examination was 49 (45.37%), 23 (21.29%), 20 (18.51%) and 16 (14.81%) buffaloes respectively. There is no significant difference observed in blood glucose and NEFA in between cyclic (47.33 ±4.64 mgdl-1and 0.18± 0.2mMolml-1) and acyclic (51.25± 3.27 mgdl-1and 0.24± 0.02 mMolml-1) buffaloes respectively. There was no significant difference observed in level of serum calcium in cyclic (10.31 ± 0.28 mgdl-1) and acyclic (9.62 ± 0.02 mgdl-1) Murrah buffaloes. However, the level of serum Pi, Mg and Ca:Pi was found to be significantly (P<0.01) higher in cyclic buffaloes (5.37± 0.14 mgdl-1, 3.66± 0.20 mgdl-1and 1.93 ± 0.02 mgdl-1) as compared to the acyclic (3.25 ± 0.02 mgdl-1, 2.43 ± 0.10 mgdl-1 and 3.02 ± 0.10 mgdl-1) buffaloes respectively. There was no significant difference observed in level of serum Manganese and Iodine in cyclic and acyclic Murrah buffaloes. The level of serum Copper, Cobalt and Zinc is found to be significantly (P<0.01) higher in cyclic buffaloes than acyclic buffaloes. The serum Fe concentration was found to be significantly (P<0.05) higher in cyclic as compared to acyclic Murrah buffaloes. The serum progesterone level was significantly (P<0.01) higher in cyclic (4.70 ±1.27 ngml-1) as compared to the acyclic (0.67± 0.02 ngml-1) Murrah buffaloes. The estrus response and conception rates in Group-I, Group-II, Group-III and Group-IV were 66.66 percent (4/6) and 50.00 percent (2/4), 50.00 percent (3/6) and 00.00 percent, 100.00 percent (6/6) and 66.66 percent, (4/6) 15.66 percent (1/6) and 100.00 percent (1/1) respectively. The progesterone level on day 0 (i.e. prior to any treatment) in true anoestrus buffaloes was 0.50 +0.00 ngml-1 . The progesterone level rose to 1.73 ± 0.65, 2.46 ± 1.52 and 2.10 ± 0.43 ngml-1 on day 7 after GnRH treatment in Group- I, Group- II and Group- III respectively. There was no change in progesterone level in buffaloes of control group. The mean estrogen level on day 0 in Group-I, Group-II, Group-III and Group-IV, was 6.00±0.00 pgml-1 and rose to 6.66±0.81and 8.7833±2.23 pg ml-1, on day 7 and day 9 respectively in Group-I and Group II. The mean estrogen level in Group-I, Group-II and Group-III on day 9 was 16.7±13.05, 14.83±9.00 and 12.16±7.55 pg ml-1 respectively. The mean value of estrogen in control group (Group-IV) showed no changes throughout the protocol. The mean insulin level on day 0 i.e. prior to any treatment in true anoestrus buffaloes in Group-I was 12.83±0.25, 12.75±0.30 0 and 7 which rose to 13.33±1.02 µUml-1 on day 9. The mean insulin level on day 0 was 19.25±3.46 and rose to 65.58±40.14 on day 7 and again declined to 24.08±6.86 µUml-1 . Similarity in Group-III the level of insulin on day 0, day 7 and day 9 was 18.75±7.65, 55.25±37.43 and 13.16±0.81 µUml-1 respectively. The mean concentration of insulin in Group-IV on day 0, 7 and 9 was 12.66±0.20, 12.50±0.00 and 12.50±0.00 µUml-1 respectively. The mean glucose level on day 0 in true anoestrus buffaloes in Group-I was 44.50 ± 3.72 on day 0 while it rose to 55.00±9.13 on day 7 and slight decline to 52.8±6.64 on day 9. The mean glucose level on day 0 was 70.33 ± 10.65 and slight decline to 61.5 ± 8.45 and 48.8±3.2 mgdl-1 on day 7 and day 9 respectively in Group II. The mean value of glucose in group III on day zero 47.66 ± 2.82 and slight decline to 46.1±2.45 day seven and remain constant 46.8 ± 7.13 mgdl-1 on day 9. The mean concentration of glucose in Group-IV on day 0, 7 and 9 was 42.50 ± 3.43, 40 ± 3.72 and 45 ± 3.54 mgdl-1 respectively.