Cytological Studies on Dermatological Disorders in Dogs with Emphasis on Treatment of Pyoderma
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Date
2013-04-12
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Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar
Abstract
The present research work was undertaken to study various aspects of
dermatological disorders especially pyoderma in dogs with the objectives of evaluating
different cytological techniques for diagnosis, the Staphylococcal organisms involved in
pyoderma in dogs with emphasis on methicillin resistant strains, the immune status of
dogs with pyoderma and the efficacy of Clindamycin alone as well as combination of
Clindamycin along with topical preparation of Silver sulphadiazene and Chlorhexidine
gluconate in the treatment of pyoderma. The efficacy of the two diagnostic techniques,
cytology and punch biopsy was compared. The results of the two techniques correlated as
far as bacterial and inflammatory diseases of the skin were concerned. Cytology was
found to be a simple and inexpensive tool for diagnosis of dermatological disorders.
Cutaneous punch biopsy was found to be a reliable diagnostic tool in the diagnosis of
neoplastic conditions and in conditions where there was ambiguity regarding etiology and
diagnosis. As for the etiology of pyoderma, S. intermedius was found in 81.81 per cent, S.
aureus in 18.18 per cent and coagulase negative staphylococci in 3.03 per cent of isolates
examined. Methicillin resistant staphylococci were observed phenotypically in 15.91 per
cent of isolates of which 5.68 per cent were S. aureus and 10.23 per cent were S.
intermedius. Six of these isolates were subjected to DNA extraction and PCR and four of
the isolates yielded 304 bp amplification encoding for mecA gene specific for methicillin
resistance. Further, no evidence of immunodeficiency was observed in dogs with
pyoderma. Therapeutic trials with Clindamycin @ 11mg /kg sid orally for 15 days and
combination of Clindamycin @ 11 mg/kg sid orally along with topical application of
Silver sulphadiazene and Chlorhexidine gluconate cream sid and bid for 15 days and the
topical preparation alone once and twice daily were used. Clindamycin alone and in
combination with the topical preparation was found to be useful in the treatment of
pyoderma (92.40-97.20% efficacy) and the topical preparation was found to be
moderately effective in pyoderma (41.98-66 % efficacy). It was thus concluded that
Clindamycin is an effective antibiotic for treatment of pyoderma in dogs and that the
topical preparation can be used as an adjunct therapy for treatment of pyoderma.
Description
Ph.D. Thesis
Keywords
crossing over, hybrids, biological phenomena, germinability, sowing, planting, oilseeds, fertilizers, developmental stages, tillage equipment