MOLECULAR CHARACTERIZATION OF PATANWADI, MARWARI AND DUMBA SHEEP BASED ON MICROSATELLITE MARKERS
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Date
2008
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Anand Agricultural University, Anand
Abstract
Microsatellite markers are markers of choice for genetic characterization of
populations. Present study attempts to genetically characterize Patanwadi, Marwari
and Dumba sheep breeds based on twenty pairs of Microsatellite markers chosen from
the list suggested by International Society for Animal Genetics (ISAG). The
microsatellites were successfully amplified in three multiplexes (Panels of 9, 5 and 6
markers) for automated fluorescence genotyping to assess genetic variability within
and between three sheep breeds. The observed number of alleles ranged from four
(CSSM 47) to fifteen (OarHH47) with total 207 alleles across three breeds. The
overall heterozygosity, PIC and Shannon Index values were 0.6270, 0.6540 and
1.6380 indicating high gene diversity. The highest observed heterozygosity was found
in Marwari and minimum in Dumba sheep breed. Genetic distance was least between
Marwari and Patanwadi and highest between Marwari and Dumba breeds.
Phylogenetic analysis indicated the Dumba breed clustered as one population
surrounded by the other two populations. In all populations high inbreeding was
indicated (mean FIS = 0.1133, FIT = 0.1311) within and among the breeds. Genetic
differentiation between breeds was low with a mean FST value of 0.0201 which
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Keywords
Animal Biotechnology, Analysis