Chemical and genetic profiling of various lentil (Lens culinaris Medik.) genotypes and their pharmacological attributes
Loading...
Date
2019-02
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
In the present investigation, 28 lentil (Lens culinaris Medik.) genotypes were evaluated for their chemical composition, biochemical profile, pharmacological attributes, nutritional potential, anti-nutritional traits, and genetic profile. GC-MS analysis of lentil methanolic extracts revealed the identification of various phytoconstituents representing about 51.76-91.43% of the total extract. Highest percentage contribution of phytoconstituents (91.43%) was observed in PL-4. Fatty acids and fatty esters were identified as the dominant constituents in addition to several minor components. The total phenolic, flavonoid, orthodihyric phenol, proanthocyanidines and tannin content was found in the range of 6.76-12.14 mg GAEg-1, 1.49-3.08 mg CAEg-1, 0.53-2.11 mg CLEg-1, 1.56-5.56 mg CAEg-1 and 2.58-7.22 mg TAEg-1 respectively. Genotype PL-259 exhibited the highest DPPH (IC50=498.83 μg/ml) and hydroxyl (IC50=599.08 μg/ml) radical scavenging activity. The maximum nitric oxide scavenging was observed in DPL-15 (IC50=419.29 μg/ml) while superoxide scavenging activity was highest in PTH (IC50=542.14 μg/ml). UPL-175 was found to exhibit maximum chelating activity (IC50=585.51). FRAP activity was found the maximum
in genotype PL-5. All lentil methanolic extracts were found to be effective against gram-positive (Staphylococcus aureus and Bacillus subtilis) and gram-negative (Salmonella typhiand Escherichia coli) bacterial strains in dose-dependent manner. Proximate composition analysis in terms of ash, crude fiber, protein, and carbohydrates content showed a rich nutritional potential of lentil genotypes. The content of macro and micro-nutrient was evaluated by AAS and their magnitude was observed to be laid in the following order: K>Mg>Na>Ca>Fe>Zn>Mn>Cu>Ni>Co. Antinutritional factors like phytic acid and trypsin inhibitors have been evaluated in all lentil genotypes. Genetic variability among lentil genotypes was determined by microsatellite markers.
Description
Keywords
null