Use of cattle dung extract as supplement for growth of bioinoculants
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Date
2015
Authors
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Publisher
CCSHAU
Abstract
The current global scenario firmly emphasizes the need to adopt eco-friendly agricultural
products for sustainable agriculture. Chemical agriculture has made an adverse impact on the soil and
the beneficial soil microbial communities. This eventually has led to high demand for organic produce
by the present-day health conscious society and sporadic attempts are being made by farmers all over
the world to detoxify the land by switching over to organic farming dispensing with chemical
fertilizers, pesticides, fungicides and herbicides Cattle dung is being used for different purposes from
ancient time because of humic compounds and fertilizing bio-elements available in it. The present
investigation has been undertaken to study the cattle dung extract as supplement for growth of
bioinoculants. The organic carbon, total nitrogen, C/N ratio, cellulose, hemicelluloses, lignin,
phosphorous, potassium and pH in cattle dung were 43.5%, 0.78%, 55.8%, 23.3%, 18.8%, 15.1%,
0.62%, 1.58% and 7.4 respectively. Cattle dung extract was tested as a growth medium for Azotobacer
chroococcum (Mac-27) and Pseudomonas (P-36) in comparison with growth in Jenson’s broth and
nutrient broth, respectively. The growth of Azotobacter chroococcum (Mac-27) and Pseudomonas sp.
(P-36) was slightly in cattle dung extract as compared to Jenson’s broth and nutrient broth respectively.
It was improved upon addition of two carbon substrates i.e. 1% glucose and 1% sucrose. The viable
count of Azotobacter chroococcum (Mac-27)and Pseudomonas sp. (P-36) were 6.29 and 6.17 log
cfu/ml of culture at 0 days which increased up to 9.91 and 9.84 log cfu/ml of culture; respectively after
30 days of enrichment and then decreased to 9.30 and 9.24 log cfu/ml; respectively at the end of
enrichment. Plant growth parameters were also positively affected by the enriched cattle dung extract
in wheat under pot house condition when test at with 100% RDF or 50% RDF and unamended cattle
dung extract. Hence, enrichment of cattle dung extract with beneficial microorganisms amended with at
different carbon substrates improved the quality of the cattle dung extract which resulted in to increase
the growth of wheat under pot house condition.
Description
Keywords
Livestock, Farmyard manure, Planting, Bacteria, Extraction, Biological development, Azotobacter, Wheats, Biological phenomena, Application methods