MYCOSYNTHESIS OF NANOPARTICLES AND ASSESSMENT OF THEIR ANTIMICROBIAL PROPERTIES
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Date
2023-09-18
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Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu
Abstract
In the present study, silver nanoparticles (AgNPs) were mycosynthesized using fruit body of Pleurotusfloridaand Agaricus bisporusas reducing and capping agent. The mycosynthesized AgNPs were characterized visually by observing change in colour of solution from light yellowish to dark brownish red, by UV-Visible Spectrophotometer for Surface plasmon resonance peak between 400-450 nm. DLS (Dynamic Light Scattering) showed the average particle size of mycosynthesized nanoparticlesto be 15.44 nm and 77.7 nm for 1:9 and 1:1 ratio of mushroom extract (Pleurotus florida) to silver nitrate respectively. FTIR(Fourier Transform Infrared Spectroscopy) analysis showed the presence of mainly( O-H), (C=O), (N-H) functional groups,amide I and II group and (C-N) stretching vibrations of aromatic ringfor the reduction of silver ions to metallic silver and acted as capping agents for the stabilization of synthesized AgNPs. SEM (Scanning Electron Microscopy) showed the shape of synthesized nanoparticles as spherical. XRD (X-Ray Diffraction) spectrum exhibited the 2θ values corresponding to face centered cubic crystal packaging of silver.The synthesized AgNPs showed effective antimicrobial activity against the test pathogenic bacterial strainsviz. Escherichia coli, Bacillus subtilis, Salmonellasp. The AgNPs synthesized using Pleurotus florida in 1:9 ratio of mushroom extract to silver nitrate showed the highest antimicrobial activity against Bacillus subtilis with 17.6 mm mean zone of inhibition at 30 µg/ml followed by Salmonella sp. and Escherichia coliandwith 16.3 mm and 16.0 mm respectively.
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Sharma, N. 2023. Mycosynthesis of Nanoparticles and Assessment of their Antimicrobial Properties, M.Sc. Thesis, SKUAST-Jammu, India.