ISOLATION AND CULTURE OF OVINE CHONDROCYTES
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Date
2021
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Abstract
Cartilage tissue engineering is one of the rapidly evolving field of biomedical engineering. Cartilage possesses limited intrinsic healing potential and therefore, clinical constructs using engineered cartilage is one of the most viable therapeutic strategy for repair of cartilage defect. Low yield of chondrocytes and limited availability of cartilage tissue remains the major challenges in the development of cartilage tissue constructs. Hence, an improved protocol for isolation and culture of chondrocytes that can yield maximum number of viable cells and maintain chondrocyte phenotype in vitro are highly rated novelties in recent research.
This study was therefore conducted to isolate and culture chondrocytes from ovine articular cartilage in vitro. Articular cartilage was collected from six apparently healthy, young sheep irrespective of age, breed and sex immediately
after sacrifice. Chondrocytes were isolated from the ovine articular cartilage tissue, cultured and characterized using Real time PCR.