Identification of biochemical traits for aphid resistance in barley (Hordeum vulgare L.)
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Date
2016
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Punjab Agricultural University, Ludhiana
Abstract
The present investigation was carried out to identify the biochemical traits responsible for aphid resistance in barley (Hordeum vulgare L.). For this study, genotypes resistant to aphid attack viz. EC-492253, EC-492255, EC-492350 and BCU-423 and susceptible towards it i.e. PL-426 were taken. The activities of defensive and antioxidative enzymes viz. polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL), superoxide dismutase (SOD), β-1, 3-glucanase and chitinase were determined in flag leaves and grains of barley genotypes during different stages of development. Various biochemical constituents i.e. total phenols, total soluble proteins, free amino acids, sugars and tannins were also estimated. The higher activities of PAL, SOD, PPO, β-1, 3-glucanase and chitinase in the flag leaves and grains of resistant genotypes showed their important role against aphid attack. The higher content of total phenols and tannins in the flag leaves and grains of resistant genotypes might attribute towards pest resistance. The higher contents of total soluble proteins, free amino acids and sugars in the flag leaves of susceptible genotype could be responsible for making it a better host for the aphids.
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