STUDIES ON INDUCED MUTAGENESIS IN SOYBEAN (Glycine max (L.) Merrill)
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Date
2002
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University of Agricultural Science, Dharwad
Abstract
"An investigation was carried out to elucidate information on
induced mutations in three soybean genotypes viz., Ankur, JS-335 and
Local black soybean. Two mutagens viz., gamma irradiation (10, 20 and
30 kR) and EMS (0.4, 0.6 and 0.8%) were used. Mi, M2 and M3
generations were evaluated during 1997 to 1999 at MRS farm, University
of Agricultural Sciences, Dhanvad.
Mutagenic sensitivity in Mi generation on the basis of reduced
germination, survival and pollen fertility revealed a dose dependent
reaction and differential response of the varieties. LD50 was found to be
around 20-30 kR in case of gamma irradiation and 0.4-0.6% in EMS
irrespective of the genotype. Studies in M2 generation revealed differential
response of the varieties with respect to chlorophyll and viable
mutations. EMS was more effective and efficient compared to gamma
rays.
Induced polygenic variability was assessed in M2 and M3
generations. In general, wide range of variability was created for most of
the traits in all the treated populations compared to control. The range in
general increased towards positive side in all the varieties with both the
mutagens. EMS treatments were effective in increasing the yield
potentiality compared to gamma rays in both generations.
Correlation between characters in both M2 and M3 generations, got
strengthened or weakened or altered compared to control offering better
scope for yield improvement through selection. Pods per plant exerted
maximum direct as well as indirect effects on seed yield in most of the
populations. Intergeneration correlation indicated no advantage of early
generation selection for yield.
Ten mutants of Ankur confmned their resistance to rust in field as
.well as glass house conditions. From the present investigation, 25
mutants were identified as promising for yield and yield cora.ponents
(pods per plant, 100 seed weight) and rust resistance. Ankur was more
promising in yielding useful mutants compared to JS-335 and Local
black soybean."
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