Expression of iron regulatory proteins in full-term swine placenta

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dc.contributor.authorRajamanickam, Kandasamy
dc.contributor.authorLeela, Venkatasubramanian
dc.contributor.authorSuganya, Gopalakrishnan
dc.contributor.authorTANUVAS
dc.date.accessioned2021-10-26T09:55:02Z
dc.date.available2021-10-26T09:55:02Z
dc.date.issued2020-03
dc.descriptionTNV_RDA_2020_55(8)931-942en_US
dc.description.abstractIn swine, even though the pregnant sows were with iron abundance, the inborn iron reserve of piglets was compromised. This indicates the insufficiency of molecular machinery involved in local placental iron flux. Here, we investigated the expression of iron regulatory proteins like hepcidin and ferroportin and also their association with iron reserve, inflammation and oxidative stress in placenta of full-term pregnant sows (n = 6). Amplification and sequencing of placental DNA confirmed the presence of hepcidin (MN579557) and ferroportin (MN565887) sequences and their 100% identity with existing GenBank data. Real-time amplification of placental mRNA revealed significant higher expression of hepcidin (p < .05) than ferroportin. Western blot analysis of placental tissues revealed specific bands for both hepcidin (~8 kDa) and ferroportin (~62 kDa) molecules. Immunohistochemistry revealed the immunoreactivity for both proteins in the cytoplasm and membrane of trophoblastic cells of the placenta. Hepcidin and ferroportin expressions were positively associated with placental non-haem iron reserve (p < .0001; p = .033), lipid peroxidation (p = .0060; p < .0001) and reactive oxygen species level (p = .0092; p = .0292). Hepcidin expression was positively associated with interleukin – 6 (p = .0002) and interferon gamma (p < .0001) expressions but ferroportin expression was negatively associated with interleukin-6 (p = .0005), interleukin-1β (p = .0226) and interferon gamma (p = .0059) expressions. This indicates hepcidin and ferroportin may have a role in controlling the local placental iron flux by acting as a molecular bridge between iron trafficking and inflammation.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810177328
dc.keywordsiron homeostasis, materno-foetal iron transfer, piglet anaemia, swineen_US
dc.language.isoEnglishen_US
dc.pages931-942en_US
dc.relation.ispartofseries;8
dc.subjectVeterinary Scienceen_US
dc.titleExpression of iron regulatory proteins in full-term swine placentaen_US
dc.title.alternativeReproduction in Domestic Animalsen_US
dc.typeArticleen_US
dc.volume55en_US
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