In-vitro regeneration in Gladiolus hybrida L.
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Date
2023
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Publisher
Birsa Agricultural University, Ranchi
Abstract
Gladiolus hybrida L., a perennial bulbous flowering plant belongs to monocot
family Iridaceae. It is one of the few plants which produce pleasant cut flowers
with long spikes. The application of tissue culture techniques in the
propagation of ornamental plants has become widely accepted as a standard
practice in the agricultural industry. meta-Topolin [6-(3-
hydroxybenzylamino)purine] is an aromatic cytokinin. It was first isolated from
poplar leaves. Its name is derived from “topol”, the Czech word for poplar. The
metabolism of meta-Topolin is similar to that of other cytokinins. Just as zeatin
and BAP, meta-Topolin may undergo ribosylation at position 9 without a
significant effect on the activity.
This study was conduct on G. hybrida in two variety var. Darshan and var.
Arka pratham in which among different combinations as well as concentrations
of phytohormones, highest bud breaking in Darshan was 84.3±3.72 % and in
var. Arka Pratham was 86.3±6.26 %, on MS media supplemented with 2 mg/l
meta-Topolin after 21 days of inoculation. It was 45.2±4.15 % in Darshan and
64.4±6.92 % in Arka pratham after 21 days when inoculated in MS media
supplemented with 2.0 mg/l BAP and 25 mg/l AdSO4 (established media). Bud
breaking declined when cultured for long period in the same media due to
accumulation of phenolic compound leading to darkening of culture media.
Shoot multiplication rate was slower in established media in comparison to MS
media supplemented with meta-Topolin. Rate of shoot multiplication was
found highest when explants were grown in MS media + 2 mg/l mT (6.2±1.05
shootlets per explant in Darshan and 7.06±0.80 shootlets per explant in Arka
pratham) after 45th days of inoculation. Whereas inoculation in established
media resulted in 3.46±0.73 shootlets per explant in Darshan and 3.5±0.20
shootlets per explant in Arka pratham. For root initiation, of the two
combinations, MS media + 2mg/l NAA (1-Naphthalene acetic acid) +1mg/l
BAP and MS media + 2mg/l IAA + 2g/l BAP used, maximum numbers of
roots per explant (4.6±0.20 in Darshan and 5.3±0.76 in Arka pratham) and
mean root length per explant (4.08±0.21 in Darshan and 6.56±0.75 in Arka
pratham) in NAA containing media, when derived from shootlets regenerated
in media containing 2mg/l meta-Topolin after 30 days of inoculation.
Therefore, in the present study for micro propagation of Gladiolus hybrida, MS
media supplemented with 2mg/l mT was found effective for shoot
multiplication followed by rooting in MS media supplemented with 2mg/l
NAA (1-Naphthalene acetic acid) +1mg/l BAP for both the varieties (Darshan
and Arka pratham).