Studies on growth differentiation factor-9 transcripts in in vitro matured buffalo cumulus oocyte complexes

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dc.contributor.advisorNanda, Trilok
dc.contributor.authorMalik, Hrudananda
dc.date.accessioned2017-08-18T09:15:02Z
dc.date.available2017-08-18T09:15:02Z
dc.date.issued2009
dc.description.abstractThe present study was carried out on buffalo ovarian follicles. Ovaries were obtained from slaughter house. Follicles present on the ovarian surface were counted and classified as small, medium and large follicles based on their diameter size. The follicles with diameter less than 5 mm were placed under the category of small follicles; those of diameter 5-9 mm were classified as medium follicles, whereas follicles with diameter more than 9 mm were placed under the category of large follicles. From these graded follicles cumulus oocyte complexes were aspirated and in vitro matured in TCM-199 supplemented with 10 percent FCS and 5 percent NBS and in only TCM-199 (control). In vitro matured cumulus oocyte complexes were investigated for the presence of GDF-9 gene transcript using RT-PCR analysis. After the RT-PCR analysis the amplified product was purified and cloned. The cloned product was further sequenced and analyzed to study the phylogenetic relationship of GDF-9 gene of buffalo with other species. The classification of follicle into different categories showed that the average surface count per ovary of individual class of follicle was found to be 6.80, 1.10 and 0.29 for small, medium and large follicles respectively. The overall mean COCs recovery rate was 1.3 per ovary. Based on cumulus-cell expansion, the overall maturation rate in 10 percent FCS, 5 percent NBS and control are 86.60, 76.47 and 54.79 percent respectively. RT-PCR analysis it revealed that GDF-9 transcripts were expressed in in vitro matured cumulus oocyte complexes from all grades of follicles in spite of variation in size. Upon nucleotide sequence it revealed that the sequences of GDF-9 gene is 99 percent similarity to that of Bubalus bubalis and this gene (Bubalus bubalis) clustered together with Bus taurus and separately clustered from Rattus norvegicus.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810028924
dc.keywordsFollicle, Cumulus oocyte complexes, In vitro maturationen_US
dc.language.isoenen_US
dc.publisherLUVASen_US
dc.subAnimal Biotechnologyen_US
dc.themeStudies on growth differentiation factor-9 transcripts in in vitro matured buffalo cumulus oocyte complexesen_US
dc.these.typeM.V.Sc.en_US
dc.titleStudies on growth differentiation factor-9 transcripts in in vitro matured buffalo cumulus oocyte complexesen_US
dc.typeThesisen_US
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