Prevalence and molecular epidemiological studies on trichinellosis in India
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Date
2015
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Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Abstract
The present study was conducted to determine the prevalence of trichinellosis in domestic pigs (Sus scrofa) in India. A total of 319 tissue and 279 serum samples were collected from 279 slaughter pigs. The samples were collected from small slaughter shops located in Assam, Chandigarh (Union territory), Punjab and Uttarakhand states of the country. The presence of Trichinella spp. antibodies was determined using a commercially available enzyme-linked immunosorbent assay (ELISA) kit and tissue samples were digested using the OIE recommended pepsin-HCl digestion method. The overall seroprevalence of trichinellosis was found to be 2.87% using ELISA. The age wise seroprevalence rate was found to be 2.83 % in pigs aged more than six months and 3.12 % in pigs less than six months of age. The seroprevalence was more in male (3.38%) than female (1.96%) pigs. None of the samples were found positive using Pepsin-HCl digestion method. Since none of the samples were found positive for Trichinella, PCR assay could not be employed in the current study. The age, sex and management practices of the animals were not significantly (p>0.05) associated with the infection. The research confirms that trichinellosis remains a rare zoonosis in India and extensive studies must be carried out in wildlife to identify reservoir hosts in the country.