Assessment of reproductive potential of garlic under Punjab conditions
| dc.contributor.advisor | Dhall, R.K. | |
| dc.contributor.author | Yadwinder Kaur | |
| dc.date.accessioned | 2017-11-27T04:14:49Z | |
| dc.date.available | 2017-11-27T04:14:49Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | The present study entitled “Assessment of reproductive potential of garlic (Allium sativum L.) under Punjab conditions" was conducted at Vegetable Research Farm, Department of Vegetable Science and Molecular Biology Laboratory, School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, during 2012. The first experiment was to study the effect of vernalization on reproductive potential of garlic especially on flowering and its true seed production behaviour which includes bolting behaviour, scape colour, flower colour, bulbil colour, number of bulbils per umbel, scape length and number of seeds per umbel. The thirteen garlic genotypes behaving fertile behaviour under U.S.A (long day) conditions were used in the present study and were given vernalization treatment at 4oC for two months before sowing. In control treatment, garlic bulbs were kept at room temperature before sowing. Highly significant and positive correlation was observed between number of seeds per umbel and scape length (r= 0.99); significant and positive correlation for number of seeds per umbel and number of bulbils per umbel (r=0.58); significant and positive correlation for number of seeds per umbel and scape length (r=0.61). Vernalization of garlic bulbs at 4oC (for two months) resulted in bolting, umbel formation and true seed production in nine genotypes whereas non-vernalized did not result into bolting i.e. no true seed production was observed. Out of 9 genotypes which produce true seeds after vernalization, maximum seed setting was observed in genotypes 5366, 5351 and 5477. Seed received from these genotypes were further sown but they did not germinate. These genotypes can be used in breeding programme, if these are sown under long day conditions. In another experiment, primer (AsLFY) was designed by using reference of already designed primer (gALFY-2). A total of 96 genotypes collected from different sources were used for genotype sequencing to identify SNP but only 73 genotypes among them showed good amplification. After amplification, sequencing of these 73 genotypes was done and SNP (CCGTCG) lied at 301 bp from initiation codon i.e. G for non-bolters genotypes and A for incomplete bolter genotypes and it will help in detecting incomplete bolter and non-bolter genotypes at seedling stage which can be useful for garlic breeding programmes. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810036480 | |
| dc.keywords | Seed, garlic, Alium sativum, fertility potential, SNP, vernalization | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 58 | en_US |
| dc.publisher | Punjab Agricultural University, Ludhiana | en_US |
| dc.research.problem | Assessment of reproductive potential of garlic under Punjab conditions | en_US |
| dc.sub | Vegetable Science | en_US |
| dc.subject | null | en_US |
| dc.theme | Assessment of reproductive potential of garlic | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Assessment of reproductive potential of garlic under Punjab conditions | en_US |
| dc.type | Thesis | en_US |