Molecular breeding for developing grain protein and micronutrient (Iron and Zinc) dense Indian bread wheat genotypes

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dc.contributor.advisorYadav, Neelam R.
dc.contributor.authorTomar, Luxmi
dc.date.accessioned2017-06-03T04:38:34Z
dc.date.available2017-06-03T04:38:34Z
dc.date.issued2014
dc.description.abstractMarker assisted selection strategy was used to introgress the functional Gpc-B1 allele in Indian wheat varieties (WH711 and PBW343) from Lassik, a hard red spring wheat. Fifty two of the most widely accepted Indian varieties were screened for their GPC and micronutrient (Zinc and Iron) content. A narrow range of variability was observed in GPC (9.3-13.7%), zinc (25.8-54.1mg/kg) and iron (37.5-57.9mg/kg) content. H711 and PBW343 were selected and crossed with Lassik. F2 plants were screened for presence/ absence of functional Gpc-B1 and rust resistance genes Lr37/Yr17/Sr38 and Lr34/Yr18 using allele specific amplification (ASA) markers. Plants from F2 observed, on an average 26.4% increase in GPC, 37.5% in zinc and 64.8 % in iron content. The plants did not show any rust susceptibility under field conditions. F2 plants with minimum reduction in yield were chosen for back-crossing with their respective Indian parent. Plants obtained from subsequent back crosses i.e . BC1F1 and BC1F2, also had better quality attributes than the parents. The GPC increased on an average by 30.2% and twice zinc (90.2%) and iron (52.5%) as compared to the recurrent parent. Hybrid plants also had better agronomical properties and showed resistance to stripe rust under field conditions whereas parental genotypes faced significant yield loses. These plants showed complete rust resistance due to presence of Gpc-B1/Yr36 with the Lr37/Yr17/Sr38 and Lr34/Yr18 loci. This population has all the combined benefits of better nutritional quality, yield and disease resistance and could be used both in MAS as well as for transferring the Gpc-B1in other Indian varieties to minimize the linkage drag. Though introgression of Gpc-B1 was linked with a net reduction in yield, interestingly, some of these plants with functional Gpc-B1 allele had higher TKW than either of the parents emphasizing the extent of influence of GxE interaction could have on Gpc-B1 allele. A consensus between quality and quantity has to be reached while breeding for improving GPC in wheat.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810014954
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subBiotechnology and Molecular Biology
dc.subjectwheats, planting, grain, diseases, proteins, crossing over, genetics, yields, alleles, genesen_US
dc.these.typePh.D
dc.titleMolecular breeding for developing grain protein and micronutrient (Iron and Zinc) dense Indian bread wheat genotypesen_US
dc.typeThesisen_US
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Molecular breeding for developing grain protein and micronutrient (Iron and Zinc) dense Indian bread wheat genotypes
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