Development of Dna Barcodes for a Few Important Species of Garcinia in the Central Western Ghats
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Date
2015-07
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University of Agricultural Science, Dharwad
Abstract
ABSTRACT
Garcinias are commercially gaining importance because of the presence of anti-obesity compound in the fruit rinds. DNA barcoding is relatively a new concept that has been developed for providing rapid, accurate and automatable species identification system using standardized DNA sequences as tags. In the present study attempt has been made to develop DNA barcodes for Garcinia spp. and to adopt the same to compare the phylogenetic relationship among these species as well as to develop descriptors for a few commercially important species of Garcinia. For this study, samples of five species of Garcinia namely, G. indica, G. gummi-gutta, G. morella, G. xanthochymus and G. talbotii collected from the central Western Ghats were used. Using 60 IPGRI developed descriptors of G. mangostana, morphological characterization was done for all the five species. Perhaps for the first time, novel standard descriptors were developed for G. xanthochymus, G. morella and G. talbotii. Gene regions of ITS, psbA-trnH and rbcL gene were used for developing barcode. The barcodes have been successfully developed for all the five species and has been deposited in the GenBank, National Centre for Biotechnology Information. Molecular phylogeny of these species has been constructed using the same information adopting Neighbor joining method. ITS region exhibited the highest mean interspecific distance (0.2082) than psbA-trnH and rbcL regions (0.0170 and 0.105 respectively). Best phylogenetic tree resolution was obtained using ITS gene. All the 5 species were grouped under separate clades in NJ tree of ITS gene. Two broad clusters were formed. Three species namely G. indica, G. gummi-gutta and G. morella were grouped under cluster I. G. xanthochymus and G. talbotii were grouped under
cluster II.
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