Molecular detection and characterization of porcine rotavirus from piggery units in Haryana
| dc.contributor.advisor | Bhanot, . Vandna | |
| dc.contributor.author | Sheoran, Deepika | |
| dc.date.accessioned | 2023-06-14T03:06:25Z | |
| dc.date.available | 2023-06-14T03:06:25Z | |
| dc.date.issued | 2024-04 | |
| dc.description.abstract | Porcine Rotavirus A (PRVA) belong to the family Sedoreoviridae and genus Rotavirus. It is non- enveloped virus and contains double stranded RNA genome of about 16-21 kbp in size with 5’ terminal cap and lacks poly A tail at 3’ end. Genome codes for 6 structural (VP1-4, VP6-8) and 5/6 non-structural proteins (NSP1-NSP5/6). PRVA has been reported from diarrheic and non diarrheic/asymptomatic pigs from several countries worldwide including India. However, there is no report of molecular characterisation of PRVA from Haryana. So, the present study was designed to detect the presence of PRVA in Haryana by RT-PCR targeting VP6 gene and to characterize it at molecular level. In the present study, out of the total 100 rectal swab samples, 36% (36/100) samples; (38% (19/50) diarrheic and 34% (17/50) non-diarrheic) were tested positive for PRVA by RT-PCR of partial length VP6 gene. Of the positive samples, highest positivity was observed among suckling piglets (45%) followed by weaning piglets (39.13%). None of the samples from growing piglet was found positive for PRVA. Further, the phylogenetic analysis of VP6, VP4 and VP7 genes revealed that the genotype I1, P[13], P[6], G11, G4 and combinations of G4P[6], G4P[13] and G11P[13] are circulating in pig population of Haryana. The most common combination found was G4P[6] (66.66%) followed by G11P[13] (22.22%) and G4P[13] (11.11%). The genotype G11 and the combinations G4P[13] and G11P[13] have been reported for the first time from pigs in India in the present study. The results of this study indicate co-circulation of different genotypes at the same time in pig population of Haryana and reveals high genetic diversity among different PRVA genotypes. These findings are useful for the development of more accurate diagnostic tools and provides information to understand the epidemiology of PRVA in Haryana | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810197590 | |
| dc.keywords | Porcine rotavirus, diarrheic, non-diarrheic, phylogenetic analysis, genotype, genetic diversity | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 53 + x+ I | en_US |
| dc.publisher | Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar | en_US |
| dc.sub | Veterinary Microbiology | en_US |
| dc.theme | Molecular detection and characterization of porcine rotavirus from piggery units in Haryana | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | Molecular detection and characterization of porcine rotavirus from piggery units in Haryana | en_US |
| dc.type | Thesis | en_US |
| dc.type | Thesis | en_US |
| dc.type | Thesis | en_US |