Clinico-pathological studies of caprine Paratuberculosis in Odisha
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Date
2021
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Department of Veterinary Pathology, OUAT, Bhubaneswar
Abstract
Present study was undertaken to have an in-depth study on detail epidemiological,
clinico-pathological and molecular confirmation of Mycobacterium avium subsp.
paratuberculosis in goats of Odisha. Goats randomly selected from ten different districts of
Odisha were screened for Paratuberculosis on the basis of history, clinical signs and faecal
microscopy through ZN staining. Present study recorded an overall prevalence of 38.75%
with prevalence of clinical and sub-clinical Paratuberculosis were 8.06% and 30.68%
respectively. Necropsy was conducted for eight goats that died during the study period.
Typical clinical signs observed were frequent watery diarrhoea, pasty faeces, emaciation or
lean body mass, increasingly lethargic attitude, rough body hair coat and soiled hind quarters
and perineum. Present study recorded highest occurrence in Puri district (52.29%) followed
by Ganjam district (34.88%) and Balasore district (45%). Chi-square analysis revealed a
significant higher occurrences of disease among adult goats of more than 2-year age
(51.23%), Ganjam breed (42.30%), females (39.17%), earthen floors (55.83%), rainy season
(40.37%) and goats with moderate body conditions (67.5%). Present study recorded high
occurrences of low shedders (n=219, 69.08%) followed by moderate shedders (n=64,
20.18%) and least numbers belonging to high shedders (n=34, 10.72%) of MAP bacilli during
screening. Anaemia and leucocytosis with increased lymphocyte count was consistent in
Caprine Paratuberculosis. There was significant increase in AST and ALT in Paratuberculosis
affected goats. Necropsy revealed a chronic enteropathy. Histopathological lesions were
mostly related to infiltrations of mononuclear cells. Molecular confirmation of MAP bacilli
was done from faecal as well as tissue (intestine and MLN) samples using IS900 specific
primers showing 400 bp PCR product in agarose gel electrophoresis.
Description
Th- 6686