“GREEN SYNTHESIS OF NANOPARTICLES USING PLANT EXTRACTS AND THEIR EFFECT ON RUMEN FERMENTATION IN VITRO”

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42756
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PVNR TVU AND HYDERABAD
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A study was conducted to assess the effect of green synthesized nanoparticles (NPs) on rumen fermentation in vitro. Zero valent iron (ZVI), iron oxide (IO), zinc oxide (ZnO) and copper oxide (CuO) NPs were synthesized using three different plant extracts for each NPs and characterized based on size, morphology, charge, crystallinity and associated organic compounds using transmission electron microscopy, zeta potential, X-ray diffraction, fourier transform infrared spectroscopy and thermo gravimetry. The effect of NPs on true dry matter (DM) and neutral detergent fibre (NDF) degradability, total gas and methane production, total volatile fatty acids (TVFA) concentration and protozoal populations was tested at two different concentrations (125 and 250 μg/ml) using a sorghum stover based diet as substrate through 24 h in vitro gas production technique. The ZVI and IO NPs formation was revealed by appearance of black colour while white precipitate and green colour indicated synthesis of ZnO and CuO NPs, respectively. Zeta potential of NPs was highly affected by type of NPs and less affected by type of plant extracts used for synthesis of NPs. All the NPs were crystalline in nature and chemical compounds of plant extracts acted as reducing and capping agents. Approximate size of synthesized NPs of ZVI, IO, ZnO and CuO was < 50 nm, <30 nm, 50-100 nm and < 50 nm respectively. The shape of synthesized ZVI and IO NPs was spherical and spherical and cubical, respectively while ZnO and CuO NPs had varying morphology. Organic compounds were relatively high in ZVI, IO and CuO NPs and low in ZnO-NPs. Addition of ZnO-NPs synthesized with different leaf extracts to a sorghum stover based diet increased in vitro true DM and NDF degradability, total gas production and TVFA concentration and decreased proportion of methane in the gaseous phase. Based on the results obtained, it is concluded that the ZnO-NPs synthesized using Aloe vera, Azadirachta indica and Psidium guajava plant extracts have potential to increase fibre digestion in crop-residue based ruminant diets.
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