In vitro synthesis of gingerol and analysis of expressed sequence tags for gingerol production in ginger(Zingiber officinale Rosc.)
| dc.contributor.advisor | Shylaja, M R | |
| dc.contributor.author | Manjusha, Rani | |
| dc.contributor.author | KAU | |
| dc.date.accessioned | 2021-05-07T05:09:59Z | |
| dc.date.available | 2021-05-07T05:09:59Z | |
| dc.date.issued | 2020 | |
| dc.description | PhD | en_US |
| dc.description.abstract | Black pepper (Piper nigrum L.), often described as the ‘King of spices’ is the most important spice crop, grown for its berries in the world. Indian pepper is preferred across the globe due to its intrinsic qualities. Foot rot is a devastating disease of black pepper. In the changing climate, drought can be a major threat in black pepper production. Hence, the present study was taken up at College of Horticulture, Vellanikkara and ICAR-IISR, Kozhikode to characterise and to identify superior accessions of black pepper for yield, quality and tolerance to biotic and abiotic stresses. Fifty accessions of black pepper in the bearing stage maintained in the National Active Germplasm Site of ICAR-IISR, Kozhikode formed the base material for the study. The accessions were characterised for fifty qualitative and fifty quantitative characters following the descriptor developed by IPGRI (1995). Wide variability was observed among the accessions for ten qualitative characters. Quantitative characters of shoot, leaf, spike and fruit also showed wide variability. Field tolerance to foot rot disease and pollu beetle infestation was observed among the accessions. Twenty accessions were selected from the base collection based on superiority of yield (> 450g green berries/vine) , field tolerance to foot rot disease infection (biotic susceptibility score 1) and pollu beetle infestation (biotic susceptibility score 1-3). They were further evaluated for biochemical principles of quality, tolerance to foot rot disease under artificial inoculation and tolerance to drought by physiological and biochemical analyses. Piperine, essential oil and oleoresin ranged from 3.61 - 6.96 per cent, 3.00 - 5.87 per cent and 7.10 - 11.18 per cent, respectively, across the accessions. The accessions with high value of piperine, essential oil and oleoresin were identified as 7293, 7211 and 7289 respectively. The two accessions identified viz. 7293 and 7252 contained more piperine than the highest of Panniyur 2 (6.6 per cent) reported among the released varieties . Artificial inoculation of selected accessions using Phytophthora capsici culture for screening for foot rot disease resistance based on over all disease severity index of both stem and leaf lesions showed that accession 7259 was moderately resistant. The selected accessions did not exhibit significant variation for various physiological and biochemical parameters at field capacity. However higher value of photosynthesis, chlorophyll content, chlorophyll stability index, relative water content and membrane stability index and low leaf temperature were observed for accessions viz. 7215, 7240, P 5 and 7241 after five days and ten days of moisture stress induction following field capacity compared to other accessions. Higher values of proline, SOD, catalase and peroxidase were also observed for these accessions. The visual scoring showed that accessions with higher values for most of physiological and biochemical parameters of drought tolerance viz. 7215, 7240, P5, and 7241 had lesser number of fallen leaves and more number of leaves retained at permanent wilting point (PWP). The accessions 7215 and 7240 took twenty days to reach PWP compared to eleven accessions which took only 16 days to reach PWP. Foliar nutrition with sulphate of potash, IISR - Power mix and Pink Pigmented Facultative Methylotrophs (PPFM) had positive effect on drought tolerance for the accessions (7215, 7240, P5 and 7241) having natural tolerance. The identified accessions with high yield , quality and tolerance to biotic or abiotic stress can be used for further breeding programme. | en_US |
| dc.identifier.citation | 174846 | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810167578 | |
| dc.keywords | Plant Biotechnology and Molecular Biology | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 228p. | en_US |
| dc.publisher | Centre for plant biotechnology and molecular biology,College of Horticulture, Vellanikkara | en_US |
| dc.sub | Plant Biotechnology | en_US |
| dc.theme | Gingerol and analysis of expressed sequence tags for gingerol production in ginger | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | In vitro synthesis of gingerol and analysis of expressed sequence tags for gingerol production in ginger(Zingiber officinale Rosc.) | en_US |
| dc.type | Thesis | en_US |