Preparation and Characterization of Dog Erythrocytic Membrane Antigen
Loading...
![Thumbnail Image](assets/images/Item.jpg)
Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Dog erythrocytic membrane antigen plays a major role for determining blood group. Structural and
molecular characterization of erythrocytic membrane antigen improves the production of blood
typing antisera, to study the auto antibody production in canine autoimmune haemolyticanemia.
The proteins in the lipid domain arranged from the inside of the erythrocyte to the outside. The
integral membrane proteins include membrane protein 3 visible in coomassie Brilliant blue-stained
polyacrylamide gels. The erythrocyte cytoskeleton consists of spectrin, ankyrin, actin, and protein
4.1 form a filamentous network under the lipid bilayer of erythroctic membrane. The cytoskeletal
proteins interact with integral proteins and lipids of the bilayer forms network for maintaining
membrane integrity. The external clustering of membrane protein 3 creates a recognition site for
auto antibodies. The auto antibodies from the erythrocytes of dogs with acute immune haemolytic
anemia have similar electrophoretic mobility to the human erythrocyte Rh related proteins and
glycoproteins and able to immunoprecipitate membrane proteins that on SDS-PAGE and western
blotting. The DEA 1 blood group system consists of antigens 1.1, 1.2, 1.3 which are most
significant in terms of transfusion reactions and alloantibodies produced against DEA 3, 5 and 7 are
not much clinical significance. Blood samples collected from registered donor dogs and RBC
membrane were isolated by hypotonic freeze thaw method. The ghost membrane and cytoplasmic
marker were identified in western blot.
Description
TNV_JALSI_2020_23(10)26-35
Keywords
Veterinary Science