Isolation and Screening of Arbuscular Mycorrhizal Fungi for Suppression of Orobanche sp., a Parasitic Weed in Tobacco (Nicotiana tabacum L.)
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Date
2017-10
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University of Agricultural Science, Dharwad
Abstract
An investigation was carried out to isolate native arbuscular mycorrhizal fungal (AMF) isolates from the Orobanche suppressive soils of tobacco growing areas of Nippani in Belagavi district, in order to screen them for their ability to suppress obnoxious weed Orobanche as well as to promote plant growth under pot culture studies. These experiments were conducted at the Weed Control Scheme, Main Agriculture Research Station and Department of Agricultural Microbiology, University of Agricultural Sciences, Dharwad from 2014-16.
The results of the pot experiment revealed that the native AMF isolates UASDAMFT5 (Glomus fasciculatum), UASDAMFT2 (Glomus radiata), UASDAMFT6 (Glomus deserticola), UASDAMFT3 (Glomus mosseae), AMF consortium (standard) and UASDAMFT consortium (tobacco native) suppressed the Orobanche parameters and plant growth parameters of tobacco over uninoculated plants in the presence of Orobanche. Inoculation of AMF isolates significantly improved the spore count over the uninoculated plants. The maximum spore count was recorded in AMF consortium (standard) (621 spores per 50 g), UASDAMFAMFT consortium (tobacco native) (619 spores per 50 g) and native isolates viz., UASDAMFT5, UASDAMFT2, UASDAMFT6 and UASDAMFT3 (277, 268, 267 and 265 spores per 50 g respectively ) over the uninoculated control (45 spores per 50 g).
The present study has indicated that UASDAMFT5, UASDAMFT2, UASDAMFT6 and UASDAMFT3 isolates are quite promising in inhibiting Orobanche seed germination similar to AMF consortium (standard) and UASDAMFT consortium (tobacco native) under pot culture studies. Thus, it can be concluded that UASDAMFT5 and UASDAMFT2 isolates can suppress the Orobanche seed germination efficiently and these can be used as a biotic tool in the Orobanche management.