Assessment of hybrid purity in maize (zea mays L.) by using molecular markers
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Date
2019-08-07
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Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani
Abstract
Maize (Zea mays L.; 2n=20) is third most important cereal crop and referred as the “Queen of Cereals” and “Miracle Crop” due to its high productivity potential as compared to the other cereals. Maize is native to Mexico and Central America.Maize is the most important crops in the world that can be grown in diverse seasons, ecologies it is used mainly for human food, animal feed and industry.
In the present investigation, maize hybrid (Hyd.18227×Hyd.10306)along with their female parental line (Hyd.18227) and male parental line (Hyd.10306) were screened with agro morphological, biochemical and molecular markers. The cross was chosen to study the heterosis in yield related agro morphological characters of maize. F1 plants showed significant relative positive heterosis for grain yield per plant (19.88%) and positive heterobeltiosis for kernel row number (17.64).
Protein fingerprinting in maize hybrid and their parental lines was characterized on 12% SDS polyacrylamide gel. A total of 11 protein bands were recorded, of which, male and female band (Type 2 and3 markers) of Rm value 0.663 and Rm value 0.718 with molecular weight 13.236 kDa and 11.491 kDa, respectively to confirming the heterozygosity of the cross.
This work was carried out to assess the hybrid purity of maize and their parental lines by using 24 RAPD and 18 SSR markers. Out of 15 RAPD primers and five SSR makersshowed the polymorphism between the maize parental lines and remaining primer produced monomorphic banding pattern. The RAPD and SSR markers observed with polymorphic percentage 37.45 % and 25 % respectively. The average PIC value was observed 0.194 for RAPDand 0.099for SSR.The SSR primer Umc-1858 identified 64% hybrid seeds of total cross.
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