DETECTION OF Mycoplasma gallisepticum FROM FIELD SAMPLES OF LAYING CHICKEN USING PCR
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Int. Journal of Science, Environment and Technology
Abstract
Mycoplasma gallisepticum is the most important and infectious mycoplasmosis. It
causes severe economic losses to poultry farmers in Namakkal district of Tamil Nadu. Sixty
five commercial layer flocks with lesions suggestive of Mycoplasma gallisepticum were
investigated during the study period of May 2014 to April 2015. The samples such as choanal
cleft swab, tracheal swab, trachea, lungs and air sacs were inoculated into frey’s broth
medium and these were subjected to polymerase chain reaction and was confirmed using
species specific primer. Out of these 65 farms, 36 farms were positive for MG. it is concluded
that, PCR assay for the detection of Mycoplasma gallisepticum infection is good and rapid
technique for diagnosis. So it is way to decrease the economic losses to poultry industry.
Description
Keywords
Veterinary Science, Veterinary Pathology