QUALITY IMPROVEMENT IN PAPAYA (Carica papaya L.) cv. ARKA PRABHATH THROUGH TILLING
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Date
2019-11-11
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UNIVERSITY OF AGRICULTURAL SCIENCES, GKVK BENGALURU
Abstract
The papaya cv. Arka Prabhath is an advanced generation gynodioecious cultivar
developed at IIHR-Bengaluru. The seeds of this variety were treated with various doses
(0 Gy, 50 Gy, 100 Gy, 250 Gy, 500 Gy and 750 Gy) of gamma radiation to study the
treatment influence on quality parameters. The radio sensitivity test on survival (%)
among M1 progenies showed that highest survival per cent was recorded in control
treatment (78%) followed by 66% (50 Gy), 64% (100 Gy), 72 % (250 Gy) 26% (500 Gy)
and 0 % (750 Gy). The data on nodes to first flowering ranged from 0.00 to 7.91 between
treatments. The height to first flowering ranged from 0.00 to 90.41cm, trunk
circumference 0.0 to 31.79 cm, the data on fruit yield ranged from 0.00 to 35.52 kg/tree.
M1 populations of papaya were selected and forwarded to M2 and assigned into families
(A to Y).The X family (153 days) and D family (152.6 days) took longer days to first
flowering. Significantly superior trunk circumference (33.90 cm) was observed in the A
family and M family (32.80 cm). The highest canopy spread (E-W) was observed in C
family (143.33 cm) and G family (140.50 cm). The highest yield was observed in G
family ( 57.90 kg/pt) and M family (36.40 kg/pt) .Out of 315, we identified 11 mutants to
study quality and shelf life parameters like respiration and ethylene content by down
regulating ripening related genes ACC synthase and ACC oxidase gene specific primers.
The amplified samples were pooled in 5X manner and performed HRM work through
qPCR.