Pyramiding QTLs for heat stress tolerance in bread wheat variety PBW 343
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Date
2023
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Punjab Agricultural University
Abstract
Wheat is a major cereal crop that is considered to be a source of basic calories and protein for more than 80% of the world population. Heat is the major abiotic stress limiting wheat production around the globe and its importance is expected to increase because of the expected climate change. The present study was conducted to pyramid various QTLs with complementary action for heat stress tolerance and combine them with stripe rust resistance. To accomplish these objectives, first KASP primers for SNP markers linked to grain yield and 1000-grain weight under heat stress conditions in wheat were developed. To pyramid the complementary QTLs, a double cross F2 population of 144 progenies was developed between a widely adapted variety, PBW 343 and two donor parents viz., Giza 168 and Sunstar. Based on phenotypic evaluation of the progenies under normal and heat stress conditions along with molecular marker analysis, five progenies numbered as 25, 55, 69, 79 and 94, were selected that had yield and other component traits better than the recurrent parent both under normal as well as under heat stress conditions. With the objective to combine heat tolerance with stripe rust resistance, 97 BC1F2 progenies from a cross between a rust donor parent (PBW 723) and two heat tolerant doubled haploid (DH) lines were evaluated at seedling stage for rust resistance. Ten of these 97 progenies were selected which had higher stripe rust resistance than the donor parent and grain yield than PBW 343.
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Relan, Ashima (2023). Pyramiding QTLs for heat stress tolerance in bread wheat variety PBW 343 (Unpublished Ph.D. Dissertation). Punjab Agricultural University, Ludhiana, Punjab, India.