INCLUSION OF CIDR IN SUPEROVULATORY PROTOCOL ON EMBRYO RECOVERY IN KANGAYAM COWS
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Date
2021
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Abstract
In cows, superovulation should allow the production of a maximum number of transferable embryos capable of establishing pregnancy after transfer, maintaining normal embryonic development and resuming pregnancy with the expulsion of healthy progenies of genetic superiority. A total of 16 Kangayam cows were selected and randomly divided into two equal experimental groups viz., group I and II. All the donor cows were treated with two doses of 500 μg PGF2α (i.m.) at an interval of 11 days. In group I donor cows, CIDR was inserted intravaginally on day 7 after estrus. Two mg of estradiol benzoate was injected intramuscularly at the time of CIDR insertion to all the donor cows. In group II donor cows, injection estradiol benzoate 2 mg was injected intramuscularly on day 7 after the estrus without CIDR insertion. In both the groups, from day 11, 50 mg of NIH FSH was given (i.m.) during morning and evening (twice daily) to each donor cow for a period of 4 days at an interval of 12 hours. On day 13, 500 μg of PGF2α was administered (i.m.) during morning and evening followed by CIDR was removed 12 hours after the second PGF2α injection. At 12 hours after the last FSH injection, 20 μg of inj. GnRH was given to each donor cow (i.m.). A total of 3 AIs were done at an interval of 12 hours starting at 12 hours after the GnRH injection. The embryos were collected from superovulated donors on day 7 after 1st AI by non-surgical transcervical method. A Foley catheter was fixed at the anterior 1/3rd of the uterine horn and the balloon of Foley catheter was inflated with 8 – 12 ml of air. The right uterine horn was thoroughly flushed for 3-5 times using flushing media and the same procedure was repeated for the opposite horn.