IN VITRO MUTATION STUDIES IN Syngonium podophyllum
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Date
2009-07-21
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University of Agricultural Sciences GKVK, Bangalore
Abstract
An efficient and reliable micropropagation protocol was developed and promising
variants were obtained in Syngonium, an important foliage plant, by in vitro mutagenesis.
Shoot proliferation from basal shoot explants were maximum (7.4) in MS media
supplemented with 10mg/l of BAP. Length of shoots decreased with increased
concentration of BAP. Except for plantlets derived from 20mg/l 0f BAP, all other
plantlets showed 100 per cent establishment on the field condition. The shoots obtained
with 20mg/l of BAP, failed to root.
The mutagenic treatments of basal shoot explants of Syngonium, showed
morphological variations. A general retardation in the shoot and root parameters of the
regenerated plantlets was observed with ethyl methane sulphonate treatment. Greater
variations were noticed in leaf morphology including reduction in size, length, curling of
leaf. Few leaves turned albenic (reduction in chlorophyll) and this was more evident at
higher level of EMS. Reduction in chlorophyll content and cultures turning albenic was
observed when microshoots were treated with EMS. Lower concentration of EMS (0.5
per cent) did not induce variation in chlorophyll color, but with the increase in EMS
concentration (1, 1.5 and 2 per cent) changes in chlorophyll color such as microshoots
turning light green, albenic were seen. Stunted growth was observed when microshoots
were treated with higher concentration of EMS.
The encouraging results obtained from the present investigation thus emphasize
the efficiency of in vitro mutation induction for the improvement of this important foliage
plant.
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