Correlation of insulin like growth factor-1 concentration with semen characteristics of beetal buck
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Date
2022-09
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College of Veterinary Science, Assam Agricultural University, Khanapara Campus
Abstract
A total of seventy two ejaculates, six from each of twelve adult Beetal bucks
maintained at Goat Research Station, AAU, Burnihat, collected using standard artificial
vagina and a total of thirty six blood samples, three from each of twelve Beetal bucks,
collected on the day of semen collection, were used to study the correlation of serum and
seminal plasma IGF-1 concentrations with semen characteristics. IGF-1 concentration was
estimated in serum and seminal plasma using the ELISA kit following the standard
procedure. Fresh semen attributes viz. ejaculate volume, mass motility, individual
progressive motility, sperm concentration, sperm viability, intact acrosome, sperm
abnormalities, sperm plasma membrane integrity and reactive oxygen species; and frozen
semen attributes viz. post thaw sperm motility, sperm viability, intact acrosome, sperm
abnormalities, sperm plasma membrane integrity and reactive oxygen species were
evaluated following the standard procedures.
There was a highly significant positive correlation (r=0.4243; p<0.01) between
IGF-1 concentration in serum and seminal plasma of the Beetal buck.
Insulin like growth factor-1 concentration in serum had a highly significant
positive correlation with sperm viability (r=0.554; p<0.01), acrosome integrity (r=0.527;
p<0.01), post thaw sperm motility (r=0.407; p<0.01), post thaw sperm viability (r=0.426;
p<0.01) and a significant positive correlation with post thaw acrosome integrity (r=0.333;
p<0.05) of the Beetal buck semen. Insulin like growth factor-1 concentration in serum had
a highly significant negative correlation (r=-0.458; p<0.01) with SOD activity in fresh
semen.
There was no significant correlation between IGF-1 concentration in serum and
ejaculate volume, mass motility, individual progressive motility, sperm concentration,
sperm abnormality, plasma membrane integrity and post thaw sperm abnormality, post
thaw sperm plasma membrane integrity and post thaw SOD activity
Insulin like growth factor-1 concentration in seminal plasma had a significant
positive correlation with individual progressive motility (r=0.341; p<0.05) and highly
significant positive correlation with sperm viability (r=0.527; p<0.01), acrosome integrity
(r=0.539; p<0.01), sperm plasma membrane integrity (r=0.464; p<0.01), post thaw sperm
motility (r=0.644; p<0.01), post thaw sperm viability (r=0.643; p<0.01), post thaw
acrosome integrity (r=0.487; p<0.01) and post thaw sperm plasma membrane integrity
(r=0.521; p<0.01). Insulin like growth factor-1 concentration in seminal plasma had a
highly significant negative correlation with SOD activity in fresh semen (r=-0.714;
p<0.01) and frozen semen (p<0.01) of Beetal buck.
There was no significant correlation between IGF-1 concentration in seminal
plasma and ejaculate volume, mass motility, sperm abnormality, sperm concentration and
and post thaw sperm abnormality.
IGF-1 in seminal plasma can be considered as biomarker for selection of buck for
breeding. However, further validation using a large sample size is required.