Studies on Escherichian Coli Isolated From Piglet Diarrhea With Special Reference to the Selection of Suitable Strain for the Development of Vaccine
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Date
2000
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Birsa Agricultural University, Ranchi
Abstract
The present study was undertaken with objectives of finding out the cause of piglet diarrhoea for recommending a suitable antimicrobial therapy and suggesting an appropriate control measure for its prevention to improve piglet survivability.
Out of the 125 faecal samples collected from diarrhoeic piglets, 97 samples were found positive for E.coli ( approx 78 per cent ), indicating it as the predominant cause of piglet diarrhoea. Based on serotyping, 63 out of 97 strains of E.coli were grouped into 14 serogroups. The E.coli serotypes 08, 0157 and 0101 were found to be most prevalent among the serotypes isolated in the present study. Nineteen isolates were rough variant and 15 remained untypable. Biochemical reaction indicated variation among strains. As per in vitro disk diffusion test, fluoroquinolone group of drugs along with Chloramphenicol and Cefotaxime were found to be the most effective drug irrespective of the E.coli serotypes. Keeping in view the merits and demerits of the effective drugs, Ciprofloxacin or it related compound Enrofloxacin was recommended as the drug of choice for E.coli infection in piglets.
Almost all the E.coli strains subjected to Congo-red binding test, showed intense or moderate red colonies in Congo-red medium. Consequently Congo-red was not suggested to be a suitable marker for determining the enter pathogenicity of the E. coli isolates from porcine origin.