ASSESSMENT OF IMMUNE RESPONSE TO HAEMORRHAGIC SEPTICAEMIA ALUM PRECIPITATED VACCINE IN CALVES
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Date
1993
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AAU, Anand
Abstract
Studies on immune response to haemorrhagic septicaemia alum precipitated vaccine in cow calves was undertaken at the Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand. Twenty four unvaccinated healthy cow calves of either sex between the age group of four and eight months of Livestock Research Station and Animal Nutrition Department, Veterinary College, Anand were randomly divided into four groups viz., groups I, II, III and IV, each group having six calves. The calves of groups I, II and III were vaccinated with 5 ml of haemorrhagic septicaemia alum precipitated vaccine subcutaneously once, twice and thrice, respectively. Interval between two vaccinations for groups II and III was seven days. The calves of group IV were vaccinated with 5 ml of HS alum precipitated vaccine with simultaneous treatment of levamisole hydrochloride at dose rate of 2.5 mg per kg body weight intramuscularly. The calves of each group were bled prior to the first vaccination and thereafter at monthly interval upto six months. The sera were subjected to IHA test, CFT and PMPT for assessment of immune response. In the sera of calves of group I, overall duration of immunity toy single vaccination was observed upto six months, with variations in peak levels as ascertained by different tests employed. Maximum level of mean IHA antibody titres was at months three and five with no significant difference from that at other months including prĀ«-vaccination stage. Maximum level of mean CP antibody titres was at month four with no significant difference from that at months two, three, five and six, but it differed significantly from that at month one and pre-vaccination stage. Maximum survivability of mice was observed at months one, two and four. No significant difference in IHA antibody titres was observed in sera of calves of groups II and III in comparison to group I Group mean CF antibody titres of groups I and II did not differ significantly, but the group mean CF antibody titres of group III were significantly higher than those of groups I and II. No significant difference between the survivability rate of mice of groups I, II and III was observed in PMPT. These results indicated that twice vaccination failed to increase immune response, but thrice vaccination had increased the CF antibody titre with no effect on IHA antibody and mouse protecting antibodies. In the sera of calves of group IV, levamisole treatment increased mean CF antibody titres significantly, but failed to increase IHA antibody titres and passive mouse protecting antibody in comparison to calves of group I. While comparing efficacy of different tests, it was observed that IHA titres increased significantly at month two and reached to its peak during months three to five except for drop at month four and fell back at month six close to the level of month two titre. These mean differences were statistically highly significant. No definite conclusion could be made regarding IHA titre. The mean CF titre value increased significantly at month one in relation to pre-vaccination titre. The peak level of mean CF antibody titres were'observed at months two, three and four followed by significant decline at month five and further significant decline at month six. The mean CF values observed during different intervals of post-vaccination period were significantly higher than that at pre-vaccination stage. The results of PMPT indicated that mean value for survivability rate in mice at different months of post-vaccination period were significantly higher than that at pre-vaccination stage. The values were found highest at months one and two followed by gradual decrease during months three, four, five and six.
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VERTINARY MICROBIOLOGY, ASSESSMENT