COMPARATIVE STUDY OF Pseudomonas aeruginosa, Pseudomonas fluorescens ANDPseudomonas putida ON THE GROWTH OF APPLE IN REPLANT SITE
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Date
2014
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UHF,NAUNI
Abstract
ABSTRACT
In the present study, isolation and characterization of fluorescent Pseudomonas sp. from
normal and replant site of was done. The twelve Pseudomonasisolates
were screened out for various plant growth promoting activities like siderophore, phosphate
solubilization, antifungal activity, plant growth regulators (auxins, cytokinins and gibberellins), lytic
enzymes and production of HCN and ammonia. On the basis of PGPR activities, two isolates (L and
M) were genotypically characterized by 16S rRNA gene sequencing. L showed 95% homology with
Pseudomonas putida with accession number (KF751235) and M showed 95% homology with
Pseudomonas fluorescenswith accession number (KF751236). These two strains along with another
Pseudomonas aeruginosa C strain exploited for the management of replant problem of apple in
replant site at Maggota and Sharontha (Shimla). Four different antifungal metabolites were extracted
with ethyl acetate were tentatively identified on thin layer chromatography (TLC) at Rf values 0.6,
0.62, 0.82 and 0.8. Antifungal activities of Pseudomonas sp. help in suppression of plant pathogenic
fungus in soil and these may be the reasons of better establishment of apple rootstocks treated with
fluorescent Pseudomonas strains. These two L, M and one Pseudomonas aeruginosaC strains used
in different liquid formulations to treat apple root stocks before plantation in replant site of orchards
at Maggota and Sharontha individually and their consortia for treatment of apple rootstocks before
planting. The performance of apple plants was much better in terms of root colonization capacity,
plant establishment and increase in plant growth in terms of plant height, number of nodes,
chlorophyll content of leaves and NPK of rhizosphere soil over their respective control after fifteen
months of plantation. These strains can be further exploited for management of replant problem of
apple after conducting few more field trials in replant sites and can have great importance in the field
of horticulture.
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