SCREENING OF RESTORER AND BLAST RESISTANCE GENE IN SELECTED GENOTYPE OF RICE (Oryza sativa L.) THROUGH MOLECULAR MARKERS
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Date
2013
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Birsa Agricultural University, Kanke, Ranchi, Jharkhand
Abstract
Rice (Oryza sativa L.) has two cultivated subspecies, Indica and Japonica, is one of
the leading cereal in the world and staple food for more than half the world’s population.
High-quality seeds and elite cultivars play a crucial role in its production. Hybrid rice is a
readily adoptable and viable technological option for raising the rice yield ceiling. Selected
rice hybrid has shown a 20‐30% yield advantage over pure line varieties under irrigated
conditions in many countries as well as in India. Cytoplasmic‐genetic male sterility (CMS)
combined with a fertility restoration system has been found to be the most efficient genetic
tool to exploit hybrid vigor on a commercial scale in rice. In present investigation Rf4 fertility
restorer gene was screened by using RM 6100 SSR primer in 96 genotypes. Experimental
result showed that total 39 genotypes having Rf4 fertility restorer gene and all these 39
genotypes were screened for three blast resistance genes (Pi-1, Pi-2 and Pi-kh).Blast
resistance genes Pi-1, Pi-2 and Pi-kh was screened by using SSR primers RM 224, RM 527
and RM 144 respectively. Screening result revealed that blast resistance gene Pi-1, Pi-2 and
Pi-kh was found in 11, 18 and 4 genotypes respectively. Genotype RC 8 was carried these
three blast resistant genes. Two blast resistant genes (Pi-1 & Pi-2) were found in following
genotypes, D 100, IR 64683-87-2-2-3-3(PSB RC 82), D 4098, CT 21407-17P-3P-1SR-1. CT
18494-1-2-1-4SR-3P and SAGC-03 both genotypes have Pi- kh gene along with Pi-1 and Pi-
2 blast resistance genes respectively. Genotypes HEXI 41, CT 21423-17P-2P-1SR-2, CT
18493-2-1-1-3VI-3, CT 16658-5-2-2SR-2-3-6MP, CT 18838-1-1-2-2SR-1P have only Pi-
1blast resistant gene. Twelve genotypes i.e. ZX788, B11586-MR-11-2-2-11, IR 10L185,
IR04A117, B13137-7-MR-1, JH 15-1-1-1,HAU 565, ZHONGHOU 1, LPD 204-B-B5-12-2-
1-2, CT 2139-F3-8-2-1, B13138-4-MR-3 and SWARNA have only Pi-2 blast resistance
gene. CT 18821-3-4-3-2SR-3P has only Pi-kh blast resistance gene. Whereas fourteen
restorer genotypes, IR 10L325 , CT 19201-3-5-2VI-1, IR10L297 , IR10L321, S3382-2D-KN-
4-1 , WEED TOLERANT RICE 1, CT 21424-16P-8P-3SR-1, WAS 191-9-B-2(NERICAL-L-
57) , ZX 115 , IR 83105-B-B-2 , KCD 1 , LPD 104-B-B1-4-3-1-1, and WAS 122-IDSA-1-
WAS-B(NERICAL-L-20) and IR 42 do not have any of these three blast resistance genes.
Maximum grain yield was 71.42 q/ha in genotype IR 10L557. Test weight was
recorded as maximum in genotypes SAGC-03 (31.55 g). Highest rough rice L/B ratio was
4.52 in entry IR 09L270 and highest milled rice L/B ratio was 3.69 in entry HAU 565.
Maximum head rice recovery percentage was 81.84 % in genotype YUNIJING 23. Maximum
number of genotypes came under scale of 6 to 7 for alkali spreading. Minimum value of
water uptake in milled rice was recorded in genotype CT 18838-1-1-2-2SR-1P (55 ml). In
Phenol reaction forty one varieties gave brown colour in rough rice, In brown rice forty three
varieties gave light brown colour and fifty two varieties showed no change in colour .
Description
SCREENING OF RESTORER AND BLAST RESISTANCE GENE IN SELECTED GENOTYPE OF RICE (Oryza sativa L.) THROUGH MOLECULAR MARKERS
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