Bioefficacy of biopesticides and novel insecticides to Leucinodes orbonalis Guenee and their safety to natural enemies

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Date
2018-07
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CSKHPKV, Palampur
Abstract
Studies on the bioefficacy of biopesticides and novel insecticides to L. orbonalis and their safety to natural enemies was undertaken at CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur. The lethal concentrations computed against first instar larvae of L. orbonalis revealed cyantraniliprole as most effective with LC50 and LC90 values of 0.18 and 0.95 ppm followed by chlorantraniliprole (1.24 & 8.64 ppm), thiacloprid (14.43 & 95.09 ppm), indoxacarb (19.99 & 106.71), lambda-cyhalothrin (38.62 & 207.78 ppm), azadirachtin (57.06 & 316.34 ppm), melia (73.82 & 378.09 ppm) and eupatorium (97.84 & 477.85 ppm). Under bioefficacy studies, chlorantraniliprole resulted in minimum shoot infestation followed by cyantraniliprole, lambda-cyhalothrin, thiacloprid, indoxacarb, azadirachtin, melia and eupatorium during 2015, whereas, during 2016 the shoot infestation was minimum in cyantraniliprole followed by chlorantraniliprole, thiacloprid, lambda-cyhalothrin, indoxacarb, azadirachtin, melia and eupatorium. Fruit infestation was found minimum in chlorantraniliprole followed by cyantraniliprole, thiacloprid, lambda-cyhalothrin, indoxacarb, azadirachtin, melia and eupatorium during both the years. On the basis of index of mean persistent toxicity of 2015 & 2016, the order of effectiveness of biopesticides and novel insecticides was: chlorantraniliprole (608.34) > cyantraniliprole (580.56) > thiacloprid (519.44) > lambda cyhalothrin (409.72) > indoxacarb (297.50) > azadirachtin (256.67) > melia (213.44) > eupatorium (136.11). Studies on dissipation pattern of insecticides revealed the average recovery of 87.75 and 91.10 per cent of indoxacarb and thiacloprid, respectively through RP-HPLC. The LOD and LOQ were 0.009 and 0.03 mg/kg for indoxacarb, 0.015 and 0.05 mg/kg for thiacloprid, respectively. The initial deposit of indoxacarb and thiacloprid were 0.620±0.025 and 1.078±0.011 mg/kg during 2016 with 0.4 and 1.6 day(s) waiting period, respectively and 0.622±0.015 mg/kg and 1.133±0.044 mg/kg during 2017 with 0.4 and 1.7 day(s) waiting period, respectively. Laboratory and field studies on relative safety of all the treatments to T. chilonis showed azadirachtin, chlorantraniliprole, cyantraniliprole, melia & eupatorium safer to the parasitoid and lambda-cyhalothrin as highly toxic.
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