In vitro clonal propagation of two promising gladiolus (Gladiolus grandiflourus L.) varieties
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Date
2001
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Department of Pomology and Floriculture, College of Agriculture, Vellayani
Abstract
Studies were conducted to evolve protocol for the in vitro clonal
propagation of Gladiolus grandiflorus L. varieties during 2000-2001 in the
Department of Pomology and Floriculture, College of Agriculture, Vellayani.
Two varieties Peach Blossom and Tropic Seas were selected for the
study. Cormels were used as explant. The effects of culture medium (basal
medium, strength of MS basal medium, mode of culture, plant growth
substances, carbon sources, activated charcoal, solidifying agent, amino acids
and coconut water) and culture conditions on in vitro shoot proliferation via
enhanced release of axillary buds were studied.
MS medium supplemented with kinetin 2.00 mg I-I and NAA 0.10
mg I-I induced earliest bud initiation in both the cultivars, in the initial culture
establishment medium.
Highest shoot proliferation In both the varieties was obtained In full
strength solid MS medium supplemented with BA 4.00 mg r', NAA 0.50 mg r'
and sucrose 40.00 g r ' under light.
In vitro rooting in cultivar Peach Blossom was best obtained In MS
medium supplemented with IBA 2.00 mg r ' and sucrose 30.00 g r '. In the
cultivar Tropic Seas, in vitro rooting was best in MS medium supplemented
with IAA 2.00 mg r ' and sucrose 40.00 g r '.
The different levels of agar tried had no significant effect on multiple
shoot proliferation. Similarly activated charcoal, coconut water and amino
acids (glycine and arginine) had no beneficial effect on multiple shoot
proliferation.
Ex vitro rooting studies were not successful.
Planting out of in vitro rooted plantlets in sand soil (2 1) media
recorded a survival rate of 100.00 per cent, after 15 days.
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PG
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Citation
171986