IDENTIFICATION OF POLYMORPHISM AND EXPRESSION ANALYSIS OF SELE ASSOCIATED WITH CANINE LYMPHOMA
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Date
2021
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Abstract
A molecular study was carried out to screen for polymorphism in canine p53
and GSTT1 genes, and expression analysis of tumorigenesis markers, TK1, TK2 and MCP1 genes in dogs. A total of 69 lymphoma affected (32 animals belonging to larger breeds, 8 to smaller breeds and 29 indigenous) and 8 healthy (control) dogs were screened for polymorphism using six overlapping primer sets for complete canine p53 gene and 4 primer sets for exonic regions of GSTT1 genes. The PCR amplicons of representative samples (n=14) revealed a complete sequence homology across different genetic groups for p53 gene; while, a total of 4 variations were observed in GSTT1 gene viz., one in exon 5 (10498C>T) and three in introns (2380G>A, 10214T>C and 10324_10330 deletion). The SNP 10498C>T was found to be a non-synonymous mutation, in which 225th amino acid “proline” was replaced by “leucine”, but the structural assessment of GSTT1 protein did not reveal any variation in protein configuration.
The levels of hemoglobin and packed cell volume were found to be significantly (P<0.01) lower in lymphoma affected than in control dogs. Whereas, the were significantly higher (P<0.01) in lymphoma affected dogs than in control. The least-squares analyses of variance for the effects of SNP (10498C>T), sex, form and grade of lymphoma on hematological and biochemical parameters revealed a significant (P<0.05) influence of SNP genotype with the biochemical parameter, alkaline phosphatase; however, in respect of hematological parameters, sex of the animal was a significant (P<0.05) source of variation influencing packed cell volume.