Assessment of Immune Response Against Newcastle Disease Oral Pellet Vaccine in Desi Chicken by ELISA Test
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Date
2016-12
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Abstract
The study was carried out at Veterinary University Training and Research Centre,
Tiruchirapalli, Tamil Nadu. A total of 48 day old desi chicks obtained from a private
hatchery in Namakkal, TamilNadu were maintained under cage system of rearing up to
52 weeks of age as per standard management practices. All the 48 chicks were divided
into six groups having eight chicks in each group were subjected to different treatment
regimes. Serum samples were collected at 21 days interval and the post vaccination
antibody titre was assessed by ELISA tests. All the birds were challenged at 52 weeks of
age with 0.5 ml dose of 104.0
EID50 virulent ND field virus. All the vaccinated groups in this
study showed protective level of antibody titre throughout the study period of 52 weeks.
The challenged birds were observed for ten days for the development of clinical symptoms,
lesions and mortality. No mortality was observed in any of the vaccinated group whereas
100 percent mortality was recorded during the observation period in the unvaccinated
control groups.
Description
TNV_JPAM_2016_10(4)2821-2826