STUDIES ON IMMUNOMODULATORY EFFECT OF COW URINE DISTILLATE IN HEALTHY AND CYCLOPHOSPHAMIDE INDUCED IMMUNOSUPPRESSIVE MICE
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Date
2015
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AAU, Anand
Abstract
Modulation of immune responses to alleviate the diseases has been of interest for many years and the concept of 'Rasayana' in Ayurveda is based on related principles. Apart from being specifically stimulatory or suppressive, certain agents have been shown to possess activity to normalize or modulate pathophysiological processes and are hence called immunomodulatory agents. A large number of plant products are being investigated for immune response, modifying activity. Cow urine distillate is also being investigated for immune response modifying activity. In reference to this status, the immunomodulatory effect of Cow urine distillate in healthy and cyclophosphamide induced immunosuppressive mice have been investigated in the present study. The present study was conducted on six to eight week old swiss albimo mice which were acclimatized for five days before the start of oral dosing of Cow urine distillate and cyclophosphamide. Dose of cyclophosphamide for immunosuppression used for the study was 60 mg/kg body weight orally, once daily for 28 days. Forty eight mice were divided into eight different groups, each comprising six male mice. The mice of group I were given normal saline and served as vehicle control. Group II was administered cyclophosphamide (60 mg/kg, orally) served as immunosuppressed group control. Group III, IV and V were given Cow urine distillate (2 ml, 4 ml and 6 ml per kg), respectively. Group VI, VII and VIII were given Cow urine distillate (2 ml, 4 ml and 6 ml per kg), respectively along with cyclophosphamide (60 mg/kg, orally). Once daily oral dosing was carried out for 28 days. All the mice were monitored for any observable toxic symptoms throughout the experimental period and they were also weighed weekly to monitor body weight. Feed consumption was measured at weekly interval. Before sacrificing the mice blood samples were collected on day 29 of the study and analyzed for hematological, biochemical and immunological parameters. Organ (spleen and thymus) weighted and collected for gross and histopathological examinations.
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VETERINARY PHARMACOLOGY & TOXICOLOGY, A STUDY