Studies on haematological, Biochemical constituents and hormones in beetal goats
Abstract
The present study was conducted with the objective of establishing normal haematological, biochemical constituents and hormones in Beetal goats at various physiological stages of life. Seventy two animals at various physiological stages maintained at goat farm CCS Haryana Agricultural University were included in the investigation. The goats were divided in eight groups with male and female goats at various physiological stages viz. Pre weaning ( 6 male and 6 female), Post weaning ( 6 male and 6 female), Pre pubertal (6 male and 6 female), Post pubertal ( 6 male and 6 female), Pregnant (6 female), Non pregnant (6 female), Lactating (6 female) and Non lactating (6 female),
Blood samples were collected from animals prior to watering and feeding in morning from the jugular vein under aseptic conditions in the heparinized vials. Haematological examination for haemoglobin (Hb), packed cell volume (PCV), erythrocyte sedementation rate (ESR), total leucocyte count (TLC) and total erythrocyte count (TEC) were done immediately in fresh samples by standard techniques. Plasma was collected by centrifuging blood at 2500 rpm for 30 minutes and was stored in clean and dry glass vial with cap and was kept at -20°C till analyzed for the estimation of tri-iodothyronine (T3), thyroxin (T4), sodium, potassium, chloride, aspartate amino transferase (AST), alanine amino transferase (ALT), acid phosphatase and alkaline phosphatase.
The results revealed that the Haematological values, electrolyte and thyroid hormones were higher in males than females, whereas, enzymes were higher in females than male in Beetal goat. The Hb, TEC, TLC, PCV, T3, T4, sodium, potassium and chloride showed maximum value in pre weaning stage similarly, activity of alkaline phosphatase and acid phosphatase was also highest in pre weaning stage in both sexes compared to rest of groups in study. The value of Hb, TEC, PCV, sodium, potassium , chloride , T3, T4, and acid phosphatase did not exhibit any significant change in between post weaning, pre pubertal and post pubertal stages of study in both sexes. Lactation and pregnancy showed decreased value of Hb, TEC, PCV, sodium, potassium and chloride whereas value of T3, T4, AST, ALT, AKP and ACP increased in lactating and pregnant goat as compared to non lactating and non pregnant goat.