Effect of different culture media on developmental competence of In-vitro embryo production in cattle
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Date
2021
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ICAR-NDRI, KARNAL
Abstract
The objective of the present study was to evaluate the effect of the different embryo culture media on early embryonic developmental competence in cattle. In vitro techniques for embryo development involve many steps viz. collection of ovaries from slaughter house, collection of immature oocytes from ovaries, grading of oocytes, in vitro maturation of oocytes, in vitro sperm preparation, in vitro fertilization and culture of presumptive embryos for early embryo development. To conduct the experiments cattle ovaries were collected from Kolkata slaughterhouse and immature oocytes were collected by follicle aspiration technique. After collection, these COCs were thoroughly washed in washing media and then in maturation media before transferring them into IVM media droplets where they were cultured for 24 hours in CO2 incubator at 5% level of CO2 and at 38.5 °C with the maximum humidity. The matured oocytes in vitro were co-incubated with in vitro processed and capacitated sperm for 14-18 hr. After fertilization, presumptive zygotes were washed and cultured in in vitro culture (IVC) media. Three different culture media i.e. (TCM-199, BO-IVF and mCR2aa) were used for early embryo development. The cleavage rate was higher in TCM-199 (74.47±9.63a) and mCR2aa (71.64±10.03a) medium as compared to BO-IVF medium (53.56±3.64b). Morula development rate was significantly higher in TCM-199 (35.39±1.467c) and BO-IVF (26.83±2.18d) medium as compared to mCR2aa (22.83±2.9d) medium. Blastocyst formation rate was observed significantly higher (P<0.05) in BO-IVF medium (14.17±2.85f) compared to TCM-199 (4.63±0.6301e) but not with mCR2aa (10.72±5.42f ) culture medium. From the present study it could be concluded that all three culture medias i.e. TCM-199, BO-IVF and mCR2aa are able to produce blastocyst, but BO-IVF and mCR2aa medias have high potential to produce blastocyst.