Assessment of microbial load with special reference to Clostridium difficile from mutton and chevon samples and the antimicrobial pattern of retail outlets of Orathanadu region, Tamil Nadu
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Date
2020-02
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Abstract
A study was conducted to assess the prevalence of Clostridial infections of food animals with special
reference to Clostridium difficile, an anaerobic, spore forming, rod shaped bacterium frm January2017to
December 2018. Eighty nine (89) chevon and Seventy eight(78) mutton samples procured from meat
retail outlets of Orathanadu region of Tamil Nadu were analyzed for the bacteriological examination of
Clostridium species and the 52 isolates of each were subjected to Gram-staining, catalase test, API 20A sugar
fermentation profiles and antibiotic sensitivity test (ABST)..The presence of C. difficile in chevon and mutton
samples were identified by conventional bacteriological examination and Clostridium difficile was isolated in
23 out of 89 (29.2%) in chevon and 29 out of 78 (37.1%) mutton. The antibiotic sensitivity test was conducted
and found that the isolates were susceptible to Cefotaxim (82.5%), Amoxicillin-clavulanic acid (81.3%),
Ceftriaxone (79.3%) and Enrofloxacin (77.9%), whereas resistant to Gentamicin (87.0%),Tetracycline(
81.4%), Penicillin (72.5%) and Ciprofloxacin(73.5%). Keeping in mind the food safety, the presence of
toxigenic C. difficile isolates in goat meat and sheep meat emphasizes the need for implementataion of
screening of Clostridial organisms thereby safeguarding the human health as well as animal health.
Description
TNV_20thMVC_PP_Feb-2020_OP56
Keywords
Veterinary Science