PARENTAL POLYMORPHISM SURVEY BETWEEN DONOR RICE LINES WITH BIOTIC STRESS RESISTANCE GENES AND ELITE RECIPIENT VARIETIES USING SSR MARKERS
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Date
2011
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ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY
Abstract
The present investigation entitled “Parental polymorphism survey between donor rice
lines with biotic stress resistance genes and elite recipient varieties using SSR markers”
was undertaken with the objectives to study the polymorphism existing between biotic
stress Brown Plant Hopper, Gall midge, Bacterial Leaf Blight, Blast resistant donor parents
and recipient elite varieties by screening with 150 Hyper variable SSR markers and to find
markers which are showing high polymorphism for their use in background selection of
recurrent parents. The study also attempted to compare the genetic relatedness among the
donor and elite recipient parents.
A total of 150 Hypervariable SSR markers were analyzed with sixteen parental lines
and of which 105 markers showed polymorphism. Among 105 markers, 85 markers are
polymorphic to some of the genotypes and 20 markers are highly polymorphic to all the
genotypes. The markers HRM 10167, HRM11111, HRM11570, HRM12469, HRM14250,
HRM15727, HRM16649, HRM400, HRM19697, HRM5720, HRM6697, HRM6699,
HRM22977, HRM23146, HRM24217, HRM25796, HRM26086, HRM26213, HRM28157,
HRM28580 were observed to be highly polymorphic. The target chromosomes where the
resistance genes are located in donor rice genotypes are chromosome 6, 7, 8, 9, 11, 12. Of
the 20 polymorphic markers, 12 markers were lying on target chromosomes. These include
HRM400 and HRM19697 on chromosome 6, HRM5720 and HRM6697 on chromosome 7,
HRM6699, HRM22977 and HRM23146 on chromosome 8, HRM24217 on chromosome 9,
HRM26086 and HRM26213 on chromosome 11, HRM28157 and HRM28580 on
chromosome12.
The data obtained from SSR markers were utilized for calculation of Jaccard’s
similarity co-efficient using UPGMA cluster analysis. The similarity co-efficient ranged
from 0.15 to 1 and genotypes were grouped into two major clusters. The Polymorphic
information Content (PIC) values of 20 polymorphic primers ranged from 0.33 to 0.75 with
a mean PIC value of 0.551 and it is considered informative for genetic studies and the
polymorphism shown by the markers can be used for background selection of the recurrent parents.
Description
Keywords
rice, genes, polymorphism, genetics, diseases, chromosomes, dna, planting, selection, breeds (animals)